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. 2017 Mar 22;7(1):229.
doi: 10.1038/s41598-017-00246-0.

Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Manila clam Ruditapes philippinarum

Hongtao Nie  1   2 , Xiwu Yan  3   4 , Zhongming Huo  1 , Liwen Jiang  1 , Peng Chen  1 , Hui Liu  1 , Jianfeng Ding  1 , Feng Yang  1
Affiliations

Construction of a High-Density Genetic Map and Quantitative Trait Locus Mapping in the Manila clam Ruditapes philippinarum

Hongtao Nie et al. Sci Rep. .

Erratum in

Abstract

Genetic linkage maps are indispensable tools in a wide range of genetic and genomic research. With the advancement of genotyping-by-sequencing (GBS) methods, the construction of a high-density linkage maps has become achievable in marine organisms lacking sufficient genomic resources, such as mollusks. In this study, high-density linkage map was constructed for an ecologically and commercially important clam species, Ruditapes philippinarum. For the consensus linkage map, a total of 9658 markers spanning 1926.98 cM were mapped to 18 sex-averaged linkage groups, with an average marker distance of 0.42 cM. Based on the high-density linkage map, ten QTLs for growth-related traits and shell color were detected. The coverage and density of the current map are sufficient for us to effectively detect QTL for segregating traits, and two QTL positions were all coincident with the closest markers. This high-density genetic linkage map reveals basic genomic architecture and will be useful for comparative genomics research, genome assembly and genetic improvement of R. philippinarum and other bivalve molluscan species.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Statistics of genotyped GBS markers in eight segregation patterns.
Figure 2
Figure 2
The high-density consensus linkage map of R. philippinarum. The consensus map which contained 9658 markers in 18 linkage groups was constructed through combing the male and female linkage maps.
Figure 3
Figure 3
Growth-related QTL mapping and association analysis in R. philippinarum among all linkage groups.
Figure 4
Figure 4
Shell color QTL mapping and association analysis in R. philippinarum among all linkage groups.
See this image and copyright information in PMC

References

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