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. 2017:2017:9086034.
doi: 10.1155/2017/9086034. Epub 2017 Feb 26.

The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats

Xiangxue Ma  1 , Xiaoge Wang  2 , Nan Kang  1 , Ting Chen  3 , Haijie Ji  3 , Lin Lv  3 , Xiaolan Yin  3 , Yaxin Tian  3 , Rui Zheng  4 , Yuanzhi Duan  3 , Fengyun Wang  3 , Xudong Tang  3
Affiliations

The Effect of Tong-Xie-Yao-Fang on Intestinal Mucosal Mast Cells in Postinfectious Irritable Bowel Syndrome Rats

Xiangxue Ma et al. Evid Based Complement Alternat Med. 2017.

Abstract

Objective. To investigate the effects of Tong-Xie-Yao-Fang (TXYF) on intestinal mucosal mast cells in rats with postinfectious irritable bowel syndrome (PI-IBS). Design. PI-IBS rat models were established using a multistimulation paradigm. Then, rats were treated with TXYF intragastrically at doses of 2.5, 5.0, and 10.0 g·kg-1·d-1 for 14 days, respectively. Intestinal sensitivity was assessed based on abdominal withdrawal reflex (AWR) scores and fecal water content (FWC). Mast cell counts and the immunofluorescence of tryptase and c-Fos in intestinal mucosa were measured; and serum IL-1β, TNF-α, and histamine levels were determined. Results. AWR reactivity and FWC which were significantly increased could be observed in PI-IBS rats. Remarkably increased mast cell activation ratio in intestinal mucosa, together with increased serum TNF-α and histamine levels, could also be seen in PI-IBS rats; furthermore, PI-IBS-induced changes in mast cell activation and level of serum TNF-α and histamine could be reversed by TXYF treatment. Meanwhile, tryptase and c-Fos expression were also downregulated. Conclusion. TXYF improves PI-IBS symptoms by alleviating behavioral hyperalgesia and antidiarrhea, the underlying mechanism of which involves the inhibitory effects of TXYF on activating mucosal mast cells, downregulating tryptase and c-Fos expression, and reducing serum TNF-α and histamine levels.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Effects of TXYF on body weight, fecal water content, grasping force, AWR, and locomotor activity in PI-IBS rats. (a) Rats' body weight, (b) fecal water content, (c) grasping force, (d) water injection quantity at an AWR score of 3, and (e, f) locomotor activity in control and TXYF treated groups. Mean ± SD. n = 8. ∗P < 0.05 versus normal. #P < 0.05 versus model.
Figure 2
Figure 2
Histamine, TNF-α, and IL-1β expression in PI-IBS rats serum. Data are presented as the mean ± SD. n = 8. ∗P < 0.05; ∗∗P < 0.01 versus normal; ##P < 0.01 versus model.
Figure 3
Figure 3
Mast cells counts at each group rats' proximal colon. mean ± SD. n = 8. ∗P < 0.05 versus normal. #P < 0.05 versus model.
Figure 4
Figure 4
Mast cells were detected with toluidine blue stain (the red arrow indicated the mast cells).
Figure 5
Figure 5
Expression of MCT and c-Fos in the lamina propria and submucosa of the proximal colon by double-label immunofluorescence assays.
Figure 6
Figure 6
The fluorescent intensity of MCT and c-Fos in control and treated groups. Mean ± SD. n = 3. ∗∗P < 0.01 versus normal; ##P < 0.01 versus model.
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