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. 2017 Jan 1;13(1):5-9.
doi: 10.5152/tjbh.2016.3142. eCollection 2017 Jan.

Association of ICAM-1, VCAM-1, CYCLIN D1 and Cathepsin D with Clinicopathological Parameters in Breast Carcinoma; an Immunohistochemical Study

Özgür Külahcı  1 , H Hasan Esen  2 , Elife Asut  1 , Salim Güngör  2
Affiliations

Association of ICAM-1, VCAM-1, CYCLIN D1 and Cathepsin D with Clinicopathological Parameters in Breast Carcinoma; an Immunohistochemical Study

Özgür Külahcı et al. J Breast Health. .

Abstract

Objective: Breast carcinoma is the most common malignant tumor detected in women. The hypothesis that increased levels of adhesion molecules and Cathepsin D affect cancerous cells moving away the primary tumor and contributes to migration of the cancerous cell and may cause remote organ metastases is defended. The aim of the present study was to search the association of intracellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), Cyclin D1, cathepsin D immunohistochemically with clinicopathological parameters in the patients diagnosed with invasive ductal breast carcinoma.

Materials and methods: The pathological slides of 153 patients diagnosed with invasive ductal carcinoma were evaluated retrospectively. Three groups were created. Group 1 consisted of patients with positive lymph node metastasis and extranodal tumor invasion; Group 2 consisted of patients with positive axillary lymph node metastasis and negative extranodal tumor invasion and Group 3 consisted of the patients with negative axillary lymph node metastasis. In all groups, 20 paraffin blocks belonging to the primary tumor in the breast were stained by ICAM-1, VCAM-1, Cyclin D1 and Cathepsin D. Findings were examined by comparing with clinicopathological parameters.

Results: The highest number of metastatic axillary lymph nodes and the highest rate of cathepsin D staining were statistically found in the cases with positive axillary lymph node metastasis and extranodal tumor invasion. CerbB2 was negative in the cases with negative ICAM-1 whereas estrogen receptor and progesterone receptor were positive in the cases with positive VCAM-1.

Conclusion: The present study reveals significant results for the patients diagnosed with invasive ductal carcinoma through breast biopsy especially before mastectomy in terms of increased number of metastatic axillary lymph nodes and extranodal tumor invasion by immunohistochemical Cathepsin D stain without any additional invasive intervention. Results of the present study may contribute to monitoring and treatment of the patients in the future.

Keywords: Breast carcinoma; Cathepsin D; immunohistochemistry; metastasis.

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Conflict of interest statement

Conflict of Interest: No conflict of interest was declared by the authors.

Figures

Figure 1
Figure 1
Positive cytoplasmic staining with Cathepsin D in the tumor cells (x100)
Figure 2
Figure 2
Positive cytoplasmic staining with VCAM-1 in the tumor cells (x200)
See this image and copyright information in PMC

References

    1. Greenlee RT, Murray T, Bolden S, Wingo PA. Cancer Statistics, 2000. CA Cancer J Clin. 2000;50:7–33. https://doi.org/10.3322/canjclin.50.1.7. - DOI - PubMed
    1. Gillett C, Fantl V, Smith R, Fisher C, Bartek J, Dickson C, Barnes D, Peters G. Amplification and overexpression of cyclin D1 in breast cancerdetected by immunohistochemical staining. Cancer Res. 1994;54:1812–1817. - PubMed
    1. Velikova G, Banks RE, Gearing A, Hemingway I, Forbes MA, Preston SR, Jones M, Wyatt J, Miller K, Ward U, Al-Maskatti J, Singh SM, Ambrose NS, Primrose JN, Selby PJ. Circulating soluble adhesion moleculesE-cadherin, E-selectin, intracellular adhesion molecule-1 (ICAM-1) andvasculer cell adhesion molecule-1 (VCAM-1) in patiens vith gastric cancer. Br J Cancer. 1997;76:1398–1404. https://doi.org/10.1038/bjc.1997.569. - DOI - PMC - PubMed
    1. Fantl V, Richards MA, Smith R, Lammie GA, Johnstone G, Allen D, Gregory W, Peters G, Dickson C, Barnes DM. Gene amplification onchromosome band 11q13 and estrogen receptor status in breast cancer. Eur J Cancer. 1990;26:423–429. https://doi.org/10.1016/0277-5379(90)90009-I. - DOI - PubMed
    1. Arnold A, Papanikolaou A. Cyclin D1 in breast cancer pathogenesis. JClin Oncol. 2005;23:4215–4224. https://doi.org/10.1200/JCO.2005.05.064. - DOI - PubMed

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