Study of Loma acerinae (Microsporidia) detected from three Ponto-Caspian gobies (Gobiidae) in Ukraine

Abstract

A previously recognized Glugea-belonging Microsporidia (G. melanostomi, G. markievichi, G. naidenovi) were found in the intestinal mucosa of Neogobius melanostomus, N. fluviatilis, and Proterorhinchus semilunaris (Teleostei, Gobiidae) from lower Dnieper River and Black Sea coastal inlets, Ukraine. Light microscope observations demonstrated small spherical xenomes about 0.35 mm in diameter filled by developmental stages of parasites. Mature spores were ellipsoidal, slightly narrowed at anterior pole, sometimes ovoid or elongate in shape approximately 3.1-4.5 × 2.0-2.9 μm in size. The stained spores were elongate ovoid that are measured about 2.3-4.3 × 1.6-3.4 μm. Ultrastructural study showed that xenoma wall consisted of finely granular layer up to 350 nm wide. Numerous host-derived collagenous fibers adhered to the xenoma wall were noticed. Microtubular inclusions about 40 nm in diameter occupied the episporontal space. Mature spores displayed the polar filament arranged in one row. One to two last turns of polar filament were sharply tapered to half of diameter. The number of polar filament turns varied depending of host infected from 10 (9 + 1) in N. melanostomus to 13 (11 + 2) in N. fluviatilis and 14 (13 + 1) in P. semilunaris. The endospore thickness varied depending of host infected from 30-35 nm in case of N. fluviatiis to 40-50 nm in P. semilunaris and 60-80 nm in N. melanostomus. SSU 16S rRNA sequence analysis, blast searches, and ultrastuctural data revealed similarity of all studied microsporidians with Loma acerinae (Jirovec, 1930).

Keywords: Loma acerinae; Microsporidia; Ponto-Caspian gobies; Sequence analysis; Ultrastructure.