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. 2018 Mar;55(3):2285-2300.
doi: 10.1007/s12035-017-0481-y. Epub 2017 Mar 22.

Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

Eliana Baez-Jurado  1 , Gina Guio Vega  1 , Gjumrakch Aliev  2   3   4 , Vadim V Tarasov  5 , Paula Esquinas  6 , Valentina Echeverria  7 , George E Barreto  8   9
Affiliations

Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

Eliana Baez-Jurado et al. Mol Neurobiol. 2018 Mar.

Abstract

Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.

Keywords: Adipose tissue; Astrocytes; Conditioned medium; Mesenchymal stem cells; Neuroglobin; Scratch assay.

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