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Randomized Controlled Trial
. 2017 Feb 1;21(1):27-30.
doi: 10.5935/1518-0557.20170008.

Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality

Bernardo R L de Moura  1   2 , Mariana C A Gurgel  2 , Suelen Pp Machado  1 , Priscila A Marques  1 , Juliana R Rolim  1 , Marcelo C de Lima  1 , Lister L Salgueiro  1
Affiliations
Randomized Controlled Trial

Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality

Bernardo R L de Moura et al. JBRA Assist Reprod. .

Abstract

Objective: Hyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects.

Methods: This prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group.

Results: The diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%.

Conclusion: This study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory.

Keywords: Hyaluronidase; ICSI; cumulus- oocyte complex; oocyte denudation.

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