An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a

Abstract

We describe the construction and utilization of a new mobilizable cosmid vector. Using this vector, mobilizable libraries of bacterial DNA can be efficiently made without a need for size fractionation of target DNA. The low stability of this vector in Pseudomonas fluorescens makes it useful in a rapid strategy, which is not dependent on plasmid incompatibility, for recombining transposon-induced mutations into the bacterial chromosome.