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. 2017 Mar 23;7(1):343.
doi: 10.1038/s41598-017-00472-6.

Microbial community composition is related to soil biological and chemical properties and bacterial wilt outbreak

Rui Wang  1 , Hongchun Zhang  1 , Liguang Sun  1 , Gaofu Qi  1 , Shu Chen  1 , Xiuyun Zhao  2
Affiliations

Microbial community composition is related to soil biological and chemical properties and bacterial wilt outbreak

Rui Wang et al. Sci Rep. .

Abstract

Soil microbes play important roles in plant growth and health. Little is known about the differences of soil microbes between healthy and bacterial wilt infected soils with Ralstonia solanacearum. By Illumina-MiSeq sequencing of 16S rRNA and 18S rRNA gene amplicons, we found the soil microbial composition and diversity were distinct between healthy and bacterial wilt infected soils. Soil microbial community varied at different plant growth stages due to changes of root exudates composition and soil pH. Healthy soils exhibited higher microbial diversity than the bacterial wilt infected soils. More abundant beneficial microbes including Bacillus, Agromyces, Micromonospora, Pseudonocardia, Acremonium, Lysobacter, Mesorhizobium, Microvirga, Bradyrhizobium, Acremonium and Chaetomium were found in the healthy soils rather than the bacterial wilt infected soils. Compared to bacterial wilt infected soils, the activities of catalase, invertase and urease, as well as soil pH, available phosphorous and potassium content, were all significantly increased in the healthy soils. In a conclusion, the higher abundance of beneficial microbes are positively related the higher soil quality, including better plant growth, lower disease incidence, and higher nutrient contents, soil enzyme activities and soil pH.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1
Figure 1
Temporal trends of different bacterial phyla in healthy soils (a) and bacterial wilt infected soils (b) during the different tobacco growth stages.
Figure 2
Figure 2
Comparison of abundance of different soil bacterial genera and bacterial community between healthy and bacterial wilt infected soils. (a) and (b) Abundances of different soil bacterial genera were compared between healthy and bacterial wilt infected soils; (c) Relative abundance and hierarchical cluster analysis of bacterial genera; (d) PCoA analysis of soil bacterial community. H: healthy soils; D: bacterial wilt infected soils. Bars with asterisk (*) and double asterisks (**) indicate significant (p < 0.05) and very significant (p < 0.01) difference between healthy and bacterial wilt infected soils, respectively.
Figure 3
Figure 3
Soil fungal diversity and community structure. Temporal trends of different fungal phyla in healthy soils (a) and bacterial wilt infected soils (b) during different tobacco growth stages; (c) Abundance of different fungal genera were compared between healthy and bacterial wilt infected soils. Bars with asterisk (*) and double asterisks (**) indicate significant (p < 0.05) and very significant (p < 0.01) difference between healthy and bacterial wilt infected soils, respectively; (d) PCoA analysis of soil fungal community. H: healthy soils; D: bacterial wilt infected soils.
Figure 4
Figure 4
Canonical correspondence analysis of the relationship between microbial community structure and soil properties. (a) soil bacterial community; (b) soil fungal community. The soil properties are indicated with arrows, including soil pH, invertase, catalase, soil organic matter (SOM), available potassium (AK), nitrogen (AN), and phosphorous (AP) content. H: healthy soils; D: Bacterial wilt infected soils. The percentage of variation is explained by each axis.
See this image and copyright information in PMC

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