Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Feb 15;9(2):736-745.
eCollection 2017.

Different impairment of immune and inflammation functions in short and long-term after ischemic stroke

Wen-Xing Li  1 , Fei Qi  2 , Jia-Qian Liu  3 , Gong-Hua Li  3 , Shao-Xing Dai  3 , Tao Zhang  4 , Fei Cheng  4 , Dahai Liu  4 , Song Guo Zheng  5
Affiliations

Different impairment of immune and inflammation functions in short and long-term after ischemic stroke

Wen-Xing Li et al. Am J Transl Res. .

Abstract

Ischemic stroke therapy and prognosis outcomes largely depend on the time periods after symptom onset. This study aims to explore the difference of global gene expression profiles and impairment of biological functions between short-term and long-term after stroke onset. We compared three short-term (3 h, 5 h and 24 h) and a long-term (6-month) gene expression levels by a multi-platform microarray data integration method. RankProd was used to calculate the differentially expressed genes between stroke patients and controls. DAVID Bioinformatics Resources was utilized to determine affected biological functions. Consensus cluster and hierarchical cluster methods were employed to compare the gene expression patterns of the commonly biological functions among these four time course groups. The results showed that severe impairment of inflammation and immune related functions in 5 h and 24 h after symptom onset. However, these functions were less affected in the 3 h and the 6-month groups. In addition, several key genes (CCL20, THBS1, EREG, and IL6 et al.) were dramatically down-regulated in 5 h and 24 h groups, whereas these genes showed no change or even a slight contrary expression in 3 h or 6-month groups. This study has identified the large differences of altered immune and inflammation functions based on gene levels between short and long-term after stroke onset. The findings provide valuable insight into the clinical practice and prognosis evaluation of ischemic stroke.

Keywords: Ischemic stroke; biological functions; gene expression; immune and inflammation; time periods.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Consensus matrix of the union genes in 3 commonly biological processes. A white to blue color scale represented the consensus values.
Figure 2
Figure 2
Heatmap of commonly 3 GO Biological Processes. Four time periods are represented by different colors. The color bar showed the range of log2 (fold-change). A. The immune response; B. The defense response; C. The response to wounding.
Figure 3
Figure 3
LogFC barplot of the key genes in 3 commonly GO Biological Processes. Four time periods groups are represented by different colors.
See this image and copyright information in PMC

References

    1. Sanossian N, Ovbiagele B. Prevention and management of stroke in very elderly patients. Lancet Neurol. 2009;8:1031–1041. - PubMed
    1. Kumar S, Selim MH, Caplan LR. Medical complications after stroke. Lancet Neurol. 2010;9:105–118. - PubMed
    1. Gottesman RF, Hillis AE. Predictors and assessment of cognitive dysfunction resulting from ischaemic stroke. Lancet Neurol. 2010;9:895–905. - PMC - PubMed
    1. Musuka TD, Wilton SB, Traboulsi M, Hill MD. Diagnosis and management of acute ischemic stroke: speed is critical. Can Med Assoc J. 2015;187:887–893. - PMC - PubMed
    1. Green AR. Pharmacological approaches to acute ischaemic stroke: reperfusion certainly, neuroprotection possibly. Br J Pharmacol. 2008;153(Suppl 1):S325–338. - PMC - PubMed

LinkOut - more resources