Dietary resveratrol supplementation prevents transport-stress-impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status

Abstract

This experiment was to evaluate the effect of dietary resveratrol (Res) supplementation (0, 400 mg/kg) on growth performance, meat quality, and muscle anaerobic glycolysis and antioxidant capacity of transported broilers. A total of 360 21-day-old male Cobb broilers was randomly allotted to 2 dietary treatments (Res-free group and Res group) with 12 replicates of 15 birds each. On the morning of d 42, after a 9-hour fast, 24 birds (2 birds of each replicate) were selected from the Res-free group and then equally placed into 2 crates, and the other 12 birds (one bird of each replicate) were selected from the Res group and then placed into the other crate. All birds in the 3 crates were transported according to the following protocols: 0-hour transport of birds in the Res-free group (control group), 3-hour transport of birds in the Res-free group (T group), and 3-hour transport of birds in the Res group (T + Res group). The results showed that Res not only improved feed conversion ratio (P < 0.05) but also tended to improve birds' final body weight (P < 0.10). In the Res-free group, a 3-hour transport increased serum corticosterone concentration, muscle malondialdehyde (MDA) and lactate contents, and muscle lactate dehydrogenase (LDH) activity, while it decreased muscle glycogen content, total superoxide dismutase (T-SOD), and glutathione peroxidase (GSH-PX) activities (P < 0.05), which induced decreased breast meat quality (lower pH24h and higher drip loss and L*24 h, P < 0.05). Nevertheless, compared with the T group, Res increased muscle glycogen content and T-SOD and GSH-PX activities (P < 0.05 or P < 0.10), while it decreased muscle MDA content and LDH activity (P < 0.05), which is beneficial to the meat quality maintenance of transported broilers (lower drip loss, L*24 h, and higher pH24h, P < 0.05 or P < 0.10). This study provides the first evidence that dietary resveratrol supplementation prevents transport-stress-impaired meat quality of broilers, possibly through decreasing the muscle anaerobic glycolysis metabolism and improving the muscle antioxidant capacity.

Keywords: antioxidant capacity; broiler; meat quality; resveratrol; transport stress.

Figure 1.

Effect of dietary resveratrol supplementation on serum corticosterone (CORT) concentration of transported broilers. All measurements are presented by mean values ± SE (n = 12). Within a panel bar, mean values labeled with different letters differ significantly (P < 0.05). T = transport group; T + Res = transport + resveratrol group.

Figure 2.

Effect of dietary resveratrol supplementation on muscle glycogen and lactate contents, and lactate dehydrogenase (LDH) activity of transported broilers. All measurements are presented by mean values ± SE (n = 12). Within a panel bar, mean values labeled with different letters differ significantly (P < 0.05). T = transport group; T + Res = transport + resveratrol group.

Figure 3.

Effect of dietary resveratrol supplementation on muscle antioxidant enzyme activities and MDA content of transported broilers. All measurements are presented by mean values ± SE (n = 12). Within a panel bar, mean values labeled with different letters differ significantly (P < 0.05). T = transport group; T + Res = transport + resveratrol group; T-AOC = total antioxidant capacity; T-SOD = total superoxide dismutase; CAT = catalase; GSH-PX = glutathione peroxidase; MDA = malonaldehyde.