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. 1978 Oct;75(10):4940-3.
doi: 10.1073/pnas.75.10.4940.

Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced mutations near replication origin

L Paolozzi, R Jucker, E Calef

Mechanism of phage Mu-1 integration: nalidixic acid treatment causes clustering of Mu-1-induced mutations near replication origin

L Paolozzi et al. Proc Natl Acad Sci U S A. 1978 Oct.

Abstract

Frequencies of Mu-1-induced mutants of Escherichia coli have been compared under two different experimental conditions: cells in exponential growth and the same cells treated with nalidixic acid. The average of values obtained from the nalidixic acid-treated culture is 3 times higher than that obtained from the control. Individual ratios of the frequency of mutants in the two cultures yield decreasing values from 6 to 1, starting from the point of origin of DNA replication to the termini of DNA replication. These results are compatible with the idea that Mu-1 integrates at the replication fork.

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References

    1. Genetics. 1954 Jul;39(4):429-39 - PubMed
    1. Proc Natl Acad Sci U S A. 1963 Dec;50:1043-51 - PubMed
    1. Ann Inst Pasteur (Paris). 1954 Dec;87(6):653-73 - PubMed
    1. Annu Rev Genet. 1976;10:389-412 - PubMed
    1. J Bacteriol. 1967 Nov;94(5):1664-71 - PubMed