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. 2017 May;35(5):441-443.
doi: 10.1038/nbt.3833. Epub 2017 Mar 27.

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Zenpei Shimatani  1 , Sachiko Kashojiya  2 , Mariko Takayama  2 , Rie Terada  3 , Takayuki Arazoe  1 , Hisaki Ishii  1 , Hiroshi Teramura  1 , Tsuyoshi Yamamoto  2 , Hiroki Komatsu  2 , Kenji Miura  2 , Hiroshi Ezura  2 , Keiji Nishida  1 , Tohru Ariizumi  2 , Akihiko Kondo  1   4
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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Zenpei Shimatani et al. Nat Biotechnol. 2017 May.

Abstract

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

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Comment in

  • Base editing on the rise.
    May A. May A. Nat Biotechnol. 2017 May 9;35(5):428-429. doi: 10.1038/nbt.3871. Nat Biotechnol. 2017. PMID: 28486457 No abstract available.

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