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. 1988 Mar 25;16(6):2601-12.
doi: 10.1093/nar/16.6.2601.

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome

V Knott  1 , D J Rees, Z Cheng, G G Brownlee
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Free PMC article

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E. coli chromosome

V Knott et al. Nucleic Acids Res. .
Free PMC article

Abstract

Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB (96.5') and oriC (84') in the published physical map of E. coli. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.

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References

    1. Cell. 1984 May;37(1):67-75 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5 - PubMed
    1. J Mol Biol. 1966 Mar;16(1):118-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Oct;83(20):7826-30 - PubMed
    1. Cell. 1987 Mar 13;48(5):813-25 - PubMed

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