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. 2017 Mar-Apr;7(2):191-198.

Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice

Mohamed Eddouks  1 , Ahmed Lemhadri  1 , Morad Hebi  1 , Ahmed El Hidani  1 , Naoufel Ali Zeggwagh  2 , Bachir El Bouhali  1 , Lhoussaine Hajji  1 , Remy Burcelin  3
Affiliations

Capparis spinosa L. aqueous extract evokes antidiabetic effect in streptozotocin-induced diabetic mice

Mohamed Eddouks et al. Avicenna J Phytomed. 2017 Mar-Apr.

Abstract

Objective: As the aqueous extract of Capparis spinosa (CS) possess antidiabetic effect, he present study aims to reveal the possible mechanism of action of CS in diabetic mice.

Materials and methods: Both single and repeated oral administrations of aqueous extract of CS were performed in multi-low dose streptozotocin-induced (MLDS) diabetic mice. Euglycemic hyperinsulinemic clamp was used in association with the endogenous glucose production (perfusion rate of 3-3H glucose) to evaluate the effect of CS aqueous extract on insulin sensitivity.

Results: Our study showed that aqueous extract of CS possess a potent hypoglycaemic activity in MLDS diabetic mice. Furthermore, the analysis perfusion of 3-3H glucose demonstrated the parallel decrease of basal endogenous glucose production (EGP) with the hypoglycaemic activity. EGP was lower in CS-Treated group when compared to the control group (p<0.001). The euglycemic hyperinsulinemic clamp technique demonstrated that CS treatment improves insulin sensitivity in peripheral tissues.

Conclusion: We conclude that the antihyperglycemic effet CS is probably due to the inhibition of basal endogenous glucose production and the improvement of insulin sensitivity in MLDS diabetic mice.

Keywords: Diabetes; Insulin sensitivity; Mice; Streptozotocin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Plasma glucose levels (mM) over 6 hr after single oral administration of CS aqueous extract (20 mg/kg) in diabetic mice. Data are expressed as means   S.E.M. of n= 6 mice per group. *p<0.05, and **p<0.01 and ***p<0.001 when as to baseline values (hr 0). : Control, : Capparis spinosa
Figure 2
Figure 2
Plasma glucose levels (mM) over once daily repeated oral administration of aqueous extract of CS (20 mg/kg) for 15 days in diabetic mice. Data are expressed as means   SEM of n= 6 mice per group. *p<0.05 and **p<0.01 as compared to baseline values (Day 0). □: Control, ▓: Capparis spinosa
Figure 3
Figure 3
Endogenous glucose production (mg/kg/min) after the aqueous extract of CS infusion (20 mg/kg) in diabetic mice. Data are expressed as means   S.E.M. of n= 6 mice per group. ***p<0.001 as compared to control group
Figure 4.
Figure 4.
Glucose infusion rate (mg/kg/min) after aqueous extract of CS infusion (20 mg/kg) in diabetic mice. Data are expressed as means   SEM of n= 6 mice per group
See this image and copyright information in PMC

References

    1. Adeneye AA. The leaf and seed aqueousextract of Phyllanthus amarus improvesinsulin resistance diabetes in experimental animal studies. J Ethnopharmacol. 2012;144:705–711. - PubMed
    1. American Diabetes Association. [Diagnosis and Classification of Diabetes Mellitus]; Diabetes Care. 2014 37:81–90. - PubMed
    1. Burcelin R, Eddouks M, Maury J, Kand J, Assan R, Girard J. Excessive glucose production, rather than insulin resistance, accounts for hyperglycaemia in recent-onset streptozotocin-diabetic rats. Diabetologia. 1995;38:283–290. - PubMed
    1. Chen YI, Cheng YW, Tzeng CY, Lee YC, Chang YN, Lee SC, Tsai CC, Chen JC, Tzen JT, Chang SL. Peroxisome proliferator-activated receptor activating hypoglycemic effect of Gardenia jasminoides Ellis aqueousextract and improvement of insulin sensitivity in steroid induced insulin resistant rats. BMC Complementar Altern Med. 2014;14:10. - PMC - PubMed
    1. Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet) J Ethnopharmacol. 2002;82:97–103. - PubMed

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