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. 2017 Feb 1;18(2):577-582.
doi: 10.22034/APJCP.2017.18.2.577.

Expression of Cytokines Interleukin-2, Interleukin-4, Interleukin-10 and Transforming Growth Factor β in Gastric Adenocarcinoma Biopsies Obtained from Mexican Patients

Maria Alicia Diaz Orea  1 , Veronica Muñoz Perez, Eduardo Gómez Conde, Victor Omar Castellanos Sánchez, Rogelio Gonzalez Lopez, J Carlos Flores Alonso, M Elena Cárdenas, A Luisa Galicia, Aurelio Mendoza
Affiliations

Expression of Cytokines Interleukin-2, Interleukin-4, Interleukin-10 and Transforming Growth Factor β in Gastric Adenocarcinoma Biopsies Obtained from Mexican Patients

Maria Alicia Diaz Orea et al. Asian Pac J Cancer Prev. .

Abstract

Objective: In this study, expression of Interleukin-2, Interleukin-4, Interleukin-10 and transforming growth factor beta in diffuse and intestinal type gastric cancers from Mexican patients was assessed for use as markers of malignancy. Methods: A total of 30 biopsies from gastric adenocarcinomas, 60% diffuse, 20% intestinal and 20% mixed in type, were studied by immunohistochemistry. Results: Regarding expression of cytokines, 23% were positive for IL-2, 26.7% for IL-4, 16.6% for IL-10 and none for TGF-β. There were found Significant statistically stage differences were noted.For example, for stages I-II 100% were IL-2 positive (p = 0.009), 87.5% were IL-4 positive (p = 0.005) and 100.0% IL-10 positive (p = 0.009). Young women were more likely to suffer gastric adenocarcinoma. In biopsies of male patients with gastric cancer, there was an increased expression of IL-2 and in biopsies from female patients in IL4. There was significantly greater detection of IL-4 and IL-10 expression in stages I and II than in stages III and IV. It was also found that IL-4, IL-10 had a higher positive expression in patients biopsies with low-level differentiations than patients with well differentiated gastric cancer in which cases were undetected. Conclusions: These results suggest that positive expression of IL-4 and IL-10 may be useful as a molecular marker to distinguish stage I and II diffuse gastric cancers which can be more readily controlled.

Keywords: Gastric adenocarcinoma; Cytokines; IL2; IL4; IL10; TGFβ.

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Figures

Figure 1
Figure 1
Photograph of Gastric Cancer Diffuse is Shown the Positive Expression of Interleukin -2 (IL-2) Detected by the Immunoperoxidase Technique in Section Paraffin of Biopsies of Patients with Gastric Adenocarcinoma. Typical Expression of IL-2 is Observed in the Tumor Cells Cytoplasm Using Anti-IL2 Moab and IL-2 Signals are Reveled in Brown Precipitate. A) 40X, B) 100x
Figure 2
Figure 2
Photograph of Gastric Cancer Diffuse is Shown the IL-4 Cytokine Expression by Immunohistochemistry in Biopsies of Patients with Gastric Adenocarcinoma. Section Paraffin, were Stained with Anti- IL-4 Moab and IL-4 Signals are Revealed in Brown in the Glandular Epithelium (Brown Precipitate). A) 40X, B) 100X
Figure 3
Figure 3
Immunodetection of the Expression of IL-10 Cytokine in Section Paraffin of Biopsies of Patients with Diffuse Gastric Adenocarcinoma. is Observed High Positivity in the Cytoplasm of a Large Number of Tumor Cells by Immune Peroxidase Test Using Anti-IL-10 Moab and Signals IL-10 are Reveled in Brown Precipitate. A) 40X, B) 100X
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