doi: 10.1016/0005-2736(88)90009-0.
Precipitation of solubilized Na+/K+-ATPase by divalent cations
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- PMID: 2835103
- DOI: 10.1016/0005-2736(88)90009-0
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Precipitation of solubilized Na+/K+-ATPase by divalent cations
Biochim Biophys Acta.
.
Abstract
A method for preparation of membranous fragments of pure and highly active shark rectal gland Na+/K+-ATPase by Mn2+ precipitation of C12E8-solubilized enzyme is described. The method is rapid and inexpensive, and yields enzyme with a specific Na+/K+-ATPase activity of up to 1800 mumol/mg per h at 37 degrees C. The influence of the detergent/protein and lipid/protein ratios on the yield of membrane bound enzyme is described.
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