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. 2017 Apr;45(2):816-822.
doi: 10.1177/0300060517698618. Epub 2017 Mar 29.

Evaluation of GeneXpert MTB/RIF for detecting Mycobacterium tuberculosis in a hospital in China

Tingyu Tang  1 , Fang Liu  1 , Xiaoling Lu  1 , Qingdong Huang  1
Affiliations

Evaluation of GeneXpert MTB/RIF for detecting Mycobacterium tuberculosis in a hospital in China

Tingyu Tang et al. J Int Med Res. 2017 Apr.

Abstract

Objective To evaluate the performance of GeneXpert MTB/RIF in diagnosing pulmonary tuberculosis (TB) in China. Methods This cross-sectional study included sputum specimens of 240 suspected TB cases. Specimens were examined by light microscopy for the presence of acid-fast bacilli, which were cultured by the BACTEC MGIT 960 (M960) system and detected by the GeneXpert MTB/RIF assay. The positive rate, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and average turnaround time of methods were evaluated. Results The positive rate was 36.6% (87/238) for the GeneXpert MTB/RIF assay and 34.0% (81/238) by M960 culture, with no significant difference between methods (χ2 = 0.33, p > 0.05). According to culture results, sensitivity of the GeneXpert MTB/RIF assay was 84.0% (68/81), specificity was 87.8% (129/147), the PPV was 78.2% (68/87), and the NPV was 87.2% (129/148). The agreement for results between Gene Xpert MTB/RIF and the M960 system was 82.8% and the Kappa value was 0.73. Conclusion The GeneXpert MTB/RIF assay is a simple, rapid, and accurate test for detecting Mycobacterium tuberculosis in sputum specimens.

Keywords: China; GeneXpert MTB/RIF; Tuberculosis.

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References

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