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. 2016 Feb 15:10:1-7.
doi: 10.1016/j.btre.2016.02.002. eCollection 2016 Jun.

Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation

Uttiya Dey  1 , Soumendranath Chatterjee  2 , Naba Kumar Mondal  1
Affiliations

Isolation and characterization of arsenic-resistant bacteria and possible application in bioremediation

Uttiya Dey et al. Biotechnol Rep (Amst). .

Abstract

Ground water arsenic contamination is a widespread problem in many developing countries including Bangladesh and India. In recent years development of modern innovative technologies for the removal of arsenic from aqueous system has become an interesting topic for research. In this present study, two rod shaped Gram-positive bacteria are being reported, isolated from arsenic affected ground water of Purbasthali block of Burdwan, West Bengal, India, which can tolerate arsenate concentration up to 4500 ppm and 550 ppm of arsenite concentration. From biochemical analysis and 16S rRNA sequencing, they were identified as Bacillus sp. and Aneurinibacillus aneurinilyticus respectively. The isolates SW2 and SW4 can remove 51.45% and 51.99% of arsenite and 53.29% and 50.37% of arsenate, respectively from arsenic containing culture media. Both of the isolate can oxidize arsenite to less toxic arsenate. These two arsenic resistant bacteria can be used as a novel pathway for the bioremediation of arsenic.

Keywords: Arsenic; Arsenic resistant bacteria; Bioremediation; Purbasthali.

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Figures

Fig. 1
Fig. 1
Study area map.
Fig. 2
Fig. 2
Scanning electron micrograph of (A) isolate SW2 (control), (B) isolate Sw2 treated with arsenite, (C) isolate SW4 (control) and (D) isolate SW4 treated with arsenite.
Fig. 3
Fig. 3
Phylogenetic tree of (A) isolate SW2 and (B) isolate SW4.
Fig. 4
Fig. 4
(A) Arsenite removal by isolates SW2 and SW4, (B) arsenate removal by isolates SW2 and SW4.
Fig. 5
Fig. 5
Arsenite oxidation (A) arsenite plate without bacteria, (B) arsenite plate inoculated with isolate SW2 and (C) arsenite plate inoculated with isolate SW4.
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