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. 2017 Sep;17(5):599-605.
doi: 10.1007/s10142-017-0557-9. Epub 2017 Mar 29.

Gene structure of the pregnancy-associated glycoprotein-like (PAG-L) in the Eurasian beaver (Castor fiber L.)

Aleksandra Lipka  1   2 , Marta Majewska  3 , Grzegorz Panasiewicz  4 , Martyna Bieniek-Kobuszewska  4 , Bozena Szafranska  4
Affiliations

Gene structure of the pregnancy-associated glycoprotein-like (PAG-L) in the Eurasian beaver (Castor fiber L.)

Aleksandra Lipka et al. Funct Integr Genomics. 2017 Sep.

Abstract

The pregnancy-associated glycoprotein-like family (PAG-L) is a large group of chorionic products, expressed in the pre-placental trophoblast and later in the post-implantational chorionic epithelium, and are involved in proper placenta development and embryo-maternal interaction in eutherians. This study describes identification of the PAG-L family in the genome of the Eurasian beaver (Castor fiber L.), named CfPAG-L. We identified 7657 bp of the CfPAG-L gDNA sequence (Acc. No. KX377932), encompassing nine exons (1-9) and eight introns (A-H). The length of the CfPAG-L exons (59-200 bp) was equivalently similar to the only known counterparts of bPAG1, bPAG2, and pPAG2. The length of the CfPAG-L introns ranged 288-1937 bp and was completely different from previously known PAG introns. The exonic CfPAG-L regions revealed 50.3-72.9% homology with equivalent segments of bPAG1 and pPAG2 structure. The intronic CfPAG-L regions alignments revealed a lack of homology. Within the entire CfPAG-L gene, 31 potential single nucleotide variants (SNV: 7 transversions and 24 transitions) were predicted. The identified exonic polymorphic loci did not affect the amino acid sequence of the CfPAG-L polypeptide precursor. This is the first report describing the CfPAG-L gene sequence, structural organization, and SNVs in the Eurasian beaver, one of the largest rodents.

Keywords: APs; Aspartic proteinases; Beaver; Exon-intron structure; PAGs; SNVs.

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Conflict of interest statement

Funding

This study was supported by the National Science Centre (2012/07/N/NZ9/02050) and the University of Warmia and Mazury in Olsztyn (528–0206-881). The first author was a beneficiary of the scholarship programs co-financed by the European Social Fund: “DrINNO3 Scholarships for PhD students” (DrINNO3/85/2013) and “RIM WiM – Regional Investment in Young Scientists of Warmia and Mazury” (CIiTT/RIM WiM/2014/65).

Conflict of interest

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Exon-intron structure of the identified genomic CfPAG-L sequence. Abbreviations: SP signal peptide, AS active site sequences coding domains 1 and 2 of the catalytic cleft
See this image and copyright information in PMC

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