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Review
. 2017 Mar 30;13(3):e1006209.
doi: 10.1371/journal.ppat.1006209. eCollection 2017 Mar.

Location, location, location: Use of CRISPR-Cas9 for genome editing in human pathogenic fungi

Aaron P Mitchell  1
Affiliations
Review

Location, location, location: Use of CRISPR-Cas9 for genome editing in human pathogenic fungi

Aaron P Mitchell. PLoS Pathog. .
No abstract available

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Outcomes of Cas9-single guide RNA (sgRNA) cleavage at a genomic site.
The Cas9-sgRNA complex is able to target a double-strand break in DNA to a specific site in the genome. The break is repaired by cellular machinery to generate a mutation that alters the site, thus preventing repeated cleavage. If the cell has a template that has homology to both sides of the break (left side of panel), then the template can be used by homology-dependent repair. For genome engineering, the investigator introduces into cells a custom template along with sources of Cas9 and the relevant sgRNA to create a designer mutation. Such mutations may be small changes in nucleotide sequence or a large insertion or deletion. Alternatively, the cell can use nonhomologous end joining (right side of panel) to create small insertions or deletions at the cut site.
See this image and copyright information in PMC

References

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