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1 Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina navas.laura@inta.gob.ar.
2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
3 Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina.
4 Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Rafaela, Santa Fe, Argentina.
1 Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina navas.laura@inta.gob.ar.
2 Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
3 Instituto de Microbiología y Zoología Agrícola, Instituto Nacional de Tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina.
4 Estación Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnología Agropecuaria (INTA), Rafaela, Santa Fe, Argentina.
We report here the complete annotated 6,035,547-bp draft genome sequence of Bacillus thuringiensis INTA Fr7-4. This strain contains three cry8 and two vip1 and vip2 insecticidal toxin genes.
Amadio AF, Benintende GB, Zandomeni RO. 2009. Complete sequence of three plasmids from Bacillus thuringiensis INTA-FR7-4 environmental isolate and comparison with related plasmids from the Bacillus cereus group. Plasmid 62:172–182. doi:10.1016/j.plasmid.2009.07.005.
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Navas LE, Amadio AF, Ortiz EM, Sauka DH, Benintende GB, Berretta MF, Zandomeni RO. 2017. Complete sequence and organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 plasmid harboring insecticidal genes. J Mol Microbiol Biotechnol 27:43–54. doi:10.1159/000451056.
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Amadio AF, Navas LE, Sauka DH, Berretta MF, Benintende GB, Zandomeni RO. 2013. Identification, cloning and expression of an insecticide cry8 gene from Bacillus thuringiensis INTA Fr7-4. J Mol Microbiol Biotechnol 23:401–409. doi:10.1159/000353206.
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Navas LE, Berretta MF, Pérez MP, Amadio AF, Ortiz EM, Sauka DH, Benintende GB, Zandomeni RO. 2014. Sequence and expression of two cry8 genes from Bacillus thuringiensis INTA Fr7-4, a native strain from Argentina. J Mol Microbiol Biotechnol 24:241–248. doi:10.1159/000365929.
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Zerbino DR, Birney E. 2008. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18:821–829. doi:10.1101/gr.074492.107.
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