doi: 10.1007/BF00322461.
Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus
Affiliations
- PMID: 2836705
- DOI: 10.1007/BF00322461
Item in Clipboard
Isolation and characterization of a linear DNA plasmid from Streptomyces clavuligerus
Mol Gen Genet.
1988 Apr.
Abstract
A linear DNA plasmid (pSCL) has been isolated from Streptomyces clavuligerus by a method employing high concentrations of protease. Rate-zonal sedimentation on sucrose gradients was used to purify the plasmid. The plasmid is 12 kb in length and appears to be linked to protein at its 5' termini. A restriction endonuclease map of the plasmid for ten enzymes has been determined. Evidence for terminally repeated sequences is provided by cross-hybridization analysis.
Similar articles
-
Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6129-33. doi: 10.1073/pnas.89.13.6129. Proc Natl Acad Sci U S A. 1992. PMID: 1631099 Free PMC article.
-
Isolation and physical characterization of streptomycete plasmids.Mol Gen Genet. 1981;182(1):53-9. doi: 10.1007/BF00422766. Mol Gen Genet. 1981. PMID: 6267433
-
Characterization of a linear extrachromosomal DNA element (pBL1) isolated after interspecific mating between Streptomyces bambergiensis and S. lividans.Res Microbiol. 1992 Nov-Dec;143(9):839-45. doi: 10.1016/0923-2508(92)90071-u. Res Microbiol. 1992. PMID: 1299837
-
[Plasmids and DNA cloning in streptomycetes].Mikrobiol Zh (1978). 1983 Mar-Apr;45(2):96-107. Mikrobiol Zh (1978). 1983. PMID: 6306414 Review. Russian. No abstract available.
-
Molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks.Rev Infect Dis. 1986 Sep-Oct;8(5):682-92. doi: 10.1093/clinids/8.5.682. Rev Infect Dis. 1986. PMID: 3024288 Review.
Cited by
-
Two internal origins of replication in Streptomyces linear plasmid pFRL1.Appl Environ Microbiol. 2010 Sep;76(17):5676-83. doi: 10.1128/AEM.02905-09. Epub 2010 Jul 2. Appl Environ Microbiol. 2010. PMID: 20601502 Free PMC article.
-
Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid.Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6129-33. doi: 10.1073/pnas.89.13.6129. Proc Natl Acad Sci U S A. 1992. PMID: 1631099 Free PMC article.
-
Diversity of telomere palindromic sequences and replication genes among Streptomyces linear plasmids.Appl Environ Microbiol. 2006 Sep;72(9):5728-33. doi: 10.1128/AEM.00707-06. Appl Environ Microbiol. 2006. PMID: 16957187 Free PMC article.
-
Evolution of linear plasmids.J Mol Evol. 1992 Dec;35(6):502-13. doi: 10.1007/BF00160211. J Mol Evol. 1992. PMID: 1474604
-
Long palindromes formed in Streptomyces by nonrecombinational intra-strand annealing.Genes Dev. 2000 Jul 15;14(14):1789-96. Genes Dev. 2000. PMID: 10898793 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
