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. 1988 Apr;212(1):66-70.
doi: 10.1007/BF00322445.

Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes

S C Goshorn  1 , G A Bohach, P M Schlievert
Affiliations

Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes

S C Goshorn et al. Mol Gen Genet. 1988 Apr.

Abstract

The structural gene of streptococcal pyrogenic exotoxin type C (SPE C) was cloned from the chromosome of Streptococcus pyogenes strain T18P into Escherichia coli using pBR328 as the vector plasmid. Subcloning enabled the localization of the gene (speC) to a 1.7 kb fragment. Partially purified E. coli-derived SPE C and purified streptococcal-derived SPE C, were shown to have the same molecular weight (23,800) and biological activities. A DNA probe, prepared from cloned speC, cross-hybridized with the structural genes of SPE A and SPE B indicating relatedness at the nucleotide level. The speC-derived probe also hybridized to a fragment of CS112 bacteriophage DNA containing the phage attachment site.

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