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. 2017:2017:8367042.
doi: 10.1155/2017/8367042. Epub 2017 Mar 6.

Analgesic, Anti-Inflammatory, and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae)

Maria Christina Dos Santos Verdam  1 , Fernanda Guilhon-Simplicio  2 , Kleyton Cardoso de Andrade  2 , Karina Lorena Meira Fernandes  3 , Tallita Marques Machado  2 , Felipe Moura Araújo da Silva  4 , Mayane Pereira de Souza  4 , Hector Henrique Ferreira Koolen  5 , Cristiane da Silva Paula  6 , Beatriz Cristina Konopatzki Hirota  6 , Vinícius Bednarczuk de Oliveira  6 , Cristina Mayumi Sasaki Miyazaki  6 , Milena Kalegari  6 , Marilis Dallarmi Miguel  6 , Patricia Maria Stuelp-Campelo  3 , Obdulio Gomes Miguel  6
Affiliations

Analgesic, Anti-Inflammatory, and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae)

Maria Christina Dos Santos Verdam et al. ScientificWorldJournal. 2017.

Abstract

Background. Byrsonima is a promising neotropical genus, rich in flavonoids and triterpenes, with several proven pharmacological properties. Nevertheless, Byrsonima duckeana W. R. Anderson is an Amazonian species almost not studied. Objective. To assess the antioxidant, anti-inflammatory, and analgesic activities of Byrsonima duckeana leaves. Materials and Methods. We analyzed an ethanol extract and its fractions for polyphenol content and UHPLC-MS/MS, phosphomolybdenum, DPPH, TBARS antioxidant tests, formalin-induced pain, carrageenan-induced peritonitis, acetic acid-induced abdominal writhings, and hot plate assays. Results. All the samples showed high polyphenol content and antioxidant capacity in the phosphomolybdenum, DPPH, and TBARS tests. We identified ethyl gallate, quinic acid, gallic acid, catechin, epicatechin, quercetrin, and quercetin in the samples. B. duckeana was able to reduce leukocyte migration in the carrageenan-induced peritonitis by 43% and the licking time in the formalin test by 57%. In the acetic acid-induced writhing test, the chloroform (FCL) and ethyl acetate (FEA) fractions were the most active samples. FEA was selected for the hot plate test, where all the dosages tested (5, 50, and 200 mg·kg-1) showed significant analgesic activity. Conclusion. B. duckeana has interesting analgesic and antioxidant activities, due to its high phenolic content, especially phenolic acids.

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Conflict of interest statement

The authors declare that they do not have conflict of interests.

Figures

Figure 1
Figure 1
UHPLC-MS/MS chromatogram of the ethanolic extract of B. duckeana (EEB) showing the following compounds: quinic acid (1), gallic acid (2), unknown (3), ethyl gallate (4), catechin (5), epicatechin (6), quercetrin (7), quercetin (8), and unknown (9).
Figure 2
Figure 2
Polyphenols identified in B. duckeana.
Figure 3
Figure 3
Results of the preliminary screening of analgesic potential of Byrsonima duckeana. ∗p < 0.05 versus negative control.
Figure 4
Figure 4
Effect of crude extract and fractions of Byrsonima duckeana on acetic acid-induced abdominal writhing. EEB: crude ethanolic extract. FEA: ethyl acetate fraction. FCL: chloroform fraction. FHX: hexane fraction. ∗p < 0.05 versus negative control.
Figure 5
Figure 5
Effect of ethyl acetate fraction (FEA) of Byrsonima duckeana on the hot plate test. Maximal activity of all the dosages was observed at 90 minutes. ∗p < 0.05 versus negative control.
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References

    1. Cadirci E., Halici Z., Yayla M., et al. Blocking of urotensin receptors as new target for treatment of carrageenan induced inflammation in rats. Peptides. 2016;82:35–43. doi: 10.1016/j.peptides.2016.05.006. - DOI - PubMed
    1. Longhi-Balbinot D. T., Lanznaster D., Baggio C. H., et al. Anti-inflammatory effect of triterpene 3β, 6β, 16β-trihydroxylup-20(29)-ene obtained from Combretum leprosum Mart & Eich in mice. Journal of Ethnopharmacology. 2012;142(1):59–64. doi: 10.1016/j.jep.2012.04.013. - DOI - PubMed
    1. Batista P. A., De Paula Werner M. F., Oliveira E. C., et al. The antinociceptive effect of (-)-linalool in models of chronic inflammatory and neuropathic hypersensitivity in mice. Journal of Pain. 2010;11(11):1222–1229. doi: 10.1016/j.jpain.2010.02.022. - DOI - PubMed
    1. Ruviaro L. F., Filippin L. I. Prevalence of chronic pain in a Basic Health Unit of a middle-sized city. Revista Dor. 2012;13(2):128–131. doi: 10.1590/s1806-00132012000200006. - DOI
    1. Martins D. F., Turnes B. L., Cidral-Filho F. J., et al. Light-emitting diode therapy reduces persistent inflammatory pain: role of interleukin 10 and antioxidant enzymes. Neuroscience. 2016;324:485–495. doi: 10.1016/j.neuroscience.2016.03.035. - DOI - PubMed

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