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. 2017 Apr 3;2017(4):pdb.prot093450.
doi: 10.1101/pdb.prot093450.

Isolation of High-Molecular-Weight DNA Using Organic Solvents

Michael R Green, Joseph Sambrook

Isolation of High-Molecular-Weight DNA Using Organic Solvents

Michael R Green et al. Cold Spring Harb Protoc. .

Abstract

Perhaps the most basic of all procedures in molecular cloning is the purification of nucleic acids. The key step, the removal of proteins, can often be performed simply by extracting aqueous solutions of nucleic acids with phenol:chloroform and chloroform.

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