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. 2017 Mar;22(1):33-39.
doi: 10.15430/JCP.2017.22.1.33. Epub 2017 Mar 30.

Differential MicroRNA Expression Between Gastric Cancer Tissue and Non-cancerous Gastric Mucosa According to Helicobacter pylori Status

Jung Won Lee  1 , Nayoung Kim  2 , Ji Hyun Park  3 , Hee Jin Kim  4 , Hyun Chang  5 , Jung Min Kim  6 , Jin-Wook Kim  2 , Dong Ho Lee  2
Affiliations

Differential MicroRNA Expression Between Gastric Cancer Tissue and Non-cancerous Gastric Mucosa According to Helicobacter pylori Status

Jung Won Lee et al. J Cancer Prev. 2017 Mar.

Abstract

Background: MicroRNAs (miRNAs) are key post-translational mechanisms which can regulate gene expression in gastric carcinogenesis. To identify miRNAs responsible for gastric carcinogenesis, we compared expression levels of miRNAs between gastric cancer tissue and non-cancerous gastric mucosa according to Helicobacter pylori status.

Methods: Total RNA was extracted from the cancerous regions of formalin-fixed, paraffin-embedded tissues of H. pylori-positive (n = 8) or H. pylori-negative (n = 8) patients with an intestinal type of gastric cancer. RNA expression was analyzed using a 3,523 miRNA profiling microarray based on the Sanger miRBase. Validation analysis was performed using TaqMan miRNA assays for biopsy samples from 107 patients consisted of control and gastric cancer with or without H. pylori. And then, expression levels of miRNAs were compared according to subgroups.

Results: A total of 156 miRNAs in the aberrant miRNA profiles across the miRNA microarray showed differential expression (at least a 2-fold change, P < 0.05) in cancer tissue, compared to noncancerous mucosa in both of H. pylori-negative and -positive samples. After 10 promising miRNAs were selected, validations by TaqMan miRNA assays confirmed that two miRNAs (hsa-miR-135b-5p and hsa-miR-196a-5p) were significantly increased and one miRNA (hsa-miR-145-5p) decreased in cancer tissue compared to non-cancerous gastric mucosa at H. pylori-negative group. For H. pylori-positive group, three miRNAs (hsa-miR-18a-5p, hsa-miR-135b-5p, and hsa-miR-196a-5p) were increased in cancer tissue. hsa-miR-135b-5p and hsa-miR-196a-5p were increased in gastric cancer in both of H. pylori-negative and -positive.

Conclusions: miRNA expression of the gastric cancer implies that different but partially common gastric cancer carcinogenic mechanisms might exist according to H. pylori status.

Keywords: Gastric cancer; Helicobacter pylori; Microarray; microRNAs.

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Figures

Figure 1
Figure 1
Schematic flow of the study. miRNA, microRNA; FFPE, formal-in-fixed paraffin-embedded.
Figure 2
Figure 2
Selection of promising candidate microRNAs in Helicobacter pylori-negative and -positive gastric cancer. (A) A fold change graph of 10 microRNA (miRNA) in H. pylori negative cancer. (B) A fold change graph of 10 miRNA in H. pylori-positive cancer.
Figure 3
Figure 3
Validation by TaqMan microRNA assay. MicroRNA (miRNA) expression levels in (A) the Helicobacter pylori-negative (Hp−) and (B) H. pylori-positive (Hp+) groups. *P < 0.05, †P < 0.01, ‡P < 0.001.
Figure 4
Figure 4
Confirmed 4 dysregulated microRNAs (miRNAs) according to Helicobacter pylori status.
See this image and copyright information in PMC

References

    1. Jun JK, Choi KS, Lee HY, Suh M, Park B, Song SH, et al. Effectiveness of the Korean National Cancer Screening Program in reducing gastric cancer mortality. Gastroenterology. 2017 doi: 10.1053/j.gastro.2017.01.029. [published online ahead of print January 29, 2017] - DOI - PubMed
    1. Belair C, Darfeuille F, Staedel C. Helicobacter pylori and gastric cancer: possible role of microRNAs in this intimate relationship. Clin Microbiol Infect. 2009;15:806–12. doi: 10.1111/j.1469-0691.2009.02960.x. - DOI - PubMed
    1. Shiota S, Thrift AP, Green L, Shah R, Verstovsek G, Rugge M, et al. Clinical manifestations of Helicobacter pylori-negative gastritis. Clin Gastroenterol Hepatol. 2017 doi: 10.1016/j.cgh.2017.01.006. [published online ahead of print January 18, 2017] - DOI - PubMed
    1. Nordenstedt H, Graham DY, Kramer JR, Rugge M, Verstovsek G, Fitzgerald S, et al. Helicobacter pylori-negative gastritis: prevalence and risk factors. Am J Gastroenterol. 2013;108:65–71. doi: 10.1038/ajg.2012.372. - DOI - PMC - PubMed
    1. Matsushima K, Isomoto H, Inoue N, Nakayama T, Hayashi T, Nakayama M, et al. MicroRNA signatures in Helicobacter pylori-infected gastric mucosa. Int J Cancer. 2011;128:361–70. doi: 10.1002/ijc.25348. - DOI - PubMed

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