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. 2017 Apr 6;22(4):584.
doi: 10.3390/molecules22040584.

Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSn with the Aid of Chemometrics

Lingfang Wu  1 , Wenyi Liang  2 , Wenjing Chen  3 , Shi Li  4 , Yaping Cui  5 , Qi Qi  6 , Lanzhen Zhang  7
Affiliations

Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSn with the Aid of Chemometrics

Lingfang Wu et al. Molecules. .

Abstract

Ganoderma triterpenes (GTs) are the major secondary metabolites of Ganoderma lucidum, which is a popularly used traditional Chinese medicine for complementary cancer therapy. The present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) for the identification and quality control of G. lucidum. Fifteen samples from the Chinese provinces of Hainan, Neimeng, Shangdong, Jilin, Anhui, Henan, Yunnan, Guangxi and Fujian were analyzed by HPLC-PAD and HPLC-MSn. Forty-seven compounds were detected by HPLC, of which forty-two compounds were tentatively identified by comparing their retention times and mass spectrometry data with that of reference compounds and reviewing the literature. Ganoderic acid B, 3,7,15-trihydroxy-11,23-dioxolanost-8,16-dien-26-oic acid, lucidenic acid A, ganoderic acid G, and 3,7-oxo-12-acetylganoderic acid DM were deemed to be the marker compounds to distinguish the samples with different quality according to both CA and PCA. This study provides helpful chemical information for further research on the anti-tumor activity and mechanism of action of G. lucidum. The results proved that fingerprints combined with chemometrics are a simple, rapid and effective method for the quality control of G. lucidum.

Keywords: Ganoderma lucidum; HPLC-MSn; Similarity Analysis (SA); chemometrics; triterpenes.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Overlaid HPLC chromatograms of samples from No. S1 to S15. The common pattern (marked R) was obtained by using the Similarity Evaluation System (SES) for the Chromatographic Fingerprints of TCMs.
Figure 2
Figure 2
HPLC chromatograms of G. lucidum.
Figure 3
Figure 3
Negative mode of the HPLC-MSn chromatograms of G. lucidum.
Figure 4
Figure 4
The chemical structures of the identified compounds.
Figure 5
Figure 5
Results of cluster analysis of 15 samples.
Figure 6
Figure 6
PCA scores plots of the sample from different regions.
See this image and copyright information in PMC

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