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. 1988 Mar 31;63(2):337-41.
doi: 10.1016/0378-1119(88)90537-9.

Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus

N Okazaki  1 , Y Hibino, Y Asano, M Ohmori, N Numao, K Kondo
Affiliations

Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus

N Okazaki et al. Gene. .

Abstract

The gene coding for phenylalanine dehydrogenase [PDH; L-phenylalanine: NAD+ oxidoreductase (deaminating); EC 1.4.1.-] from Bacillus sphaericus SCRC-79a was cloned onto plasmid pUC9, and the nucleotide sequence of the 2-kb DNA region of the insert was determined. A 1143-bp open reading frame consisting of 381 codons was identified as a pdh gene coding for PDH.

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