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. 2017 Jul;102(7):e241-e244.
doi: 10.3324/haematol.2016.161604. Epub 2017 Apr 6.

Enhanced calreticulin expression in red cells of polycythemia vera patients harboring the JAK2V617F mutation

Mégane Brusson  1 , Sylvie Cochet  1 , Marjorie Leduc  2 , François Guillonneau  2 , Patrick Mayeux  2 , Thierry Peyrard  3 , Christine Chomienne  4   5 , Caroline Le Van Kim  1 , Bruno Cassinat  5 , Jean-Jacques Kiladjian  6 , Wassim El Nemer  7
Affiliations

Enhanced calreticulin expression in red cells of polycythemia vera patients harboring the JAK2V617F mutation

Mégane Brusson et al. Haematologica. 2017 Jul.
No abstract available

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Figures

Figure 1.
Figure 1.
CALR expression in red blood cell membranes of patients with polycythemia vera and in BaF3 cell lines. (A) Western-blot analysis of CALR expression in control (CT, n=11) and PV (n=19) erythrocyte membranes. β-actin (Actin) is used as a loading control. (B) Quantification of CALR expression normalized by β-actin from the 11 CT and 19 PV patients in addition to 34 ET patients (see Figure 2). Horizontal lines represent medians (0.9883, 2.023 and 0.7290, respectively). Mann-Whitney test, **P=0.0024. (C) Percentage of reticulocytes in 11 CT and 12 PV red blood cell samples, as determined by flow cytometry using thiazole orange dye (medians=0.6 and 0.65, respectively; Mann-Whitney test P=0.7489). (D) Absence of correlation between the percentage of reticulocytes in PV red blood cells and CALR expression values (Spearman correlation r=−0.5247, P=0.0781). (E) Western-blot analysis of CALR expression in membranes obtained from total red blood cells (RBCs), and reticulocyte-depleted (R−) or reticulocyte-enriched (R+) fractions from three PV patients. The percentage of reticulocytes in the three populations was determined by flow cytometry using thiazole orange dye. (F) Upper panel: western-blot analysis of CALR expression in whole lysates of BaF3-JAK2WT and -JAK2V617F cell lines; a triplicate is shown for each cell line. Lower panel: quantification of CALR expression normalized by β-actin from BaF3-JAK2WT and -JAK2V617F cell lines (n=6); Mann-Whitney test, **P=0.0022.
Figure 2.
Figure 2.
CALR expression in red blood cell membranes of patients with essential thrombocythemia. (A) Western-blot analysis of CALR expression in red blood cell membranes of 34 ET patients. β-actin (Actin) was used as a loading control. The ET group is composed of patients positive for JAK2V617F (V617F, n=16), CALR mutations (CALR, n=10) or triple-negative (Neg, n=8). (B) Comparison of CALR expression between CT and the ET patient subgroups, depending on the mutation status: JAK2V617F (V617F), CALR, triple-negative (Neg). Horizontal lines represent medians. Mann-Whitney test, *P<0.05.
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References

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