Comment
doi: 10.1016/j.tim.2017.03.009.
Epub 2017 Apr 6.
Finally, Archaea Get Their CRISPR-Cas Toolbox
Affiliations
- PMID: 28391963
- DOI: 10.1016/j.tim.2017.03.009
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Comment
Finally, Archaea Get Their CRISPR-Cas Toolbox
Trends Microbiol.
2017 Jun.
Abstract
The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.
Keywords: CRISPR-Cas; Cas9; HDR; MMEJ; NHEJ; archaea.
Copyright © 2017 Elsevier Ltd. All rights reserved.
Comment on
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Cas9-mediated genome editing in the methanogenic archaeon Methanosarcina acetivorans.Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2976-2981. doi: 10.1073/pnas.1618596114. Epub 2017 Mar 6. Proc Natl Acad Sci U S A. 2017. PMID: 28265068 Free PMC article.
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