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Comment
. 2017 Jun;25(6):430-432.
doi: 10.1016/j.tim.2017.03.009. Epub 2017 Apr 6.

Finally, Archaea Get Their CRISPR-Cas Toolbox

Uri Gophna  1 , Thorsten Allers  2 , Anita Marchfelder  3
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Finally, Archaea Get Their CRISPR-Cas Toolbox

Uri Gophna et al. Trends Microbiol. 2017 Jun.

Abstract

The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.

Keywords: CRISPR-Cas; Cas9; HDR; MMEJ; NHEJ; archaea.

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