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Comparative Study
. 1987 Oct;51(4):539-41.

Evaluation of an enzyme immunoassay for detection of antibodies to pseudorabies virus in porcine field sera

A Afshar  1 , P F Wright, G C Dulac
Affiliations
Comparative Study

Evaluation of an enzyme immunoassay for detection of antibodies to pseudorabies virus in porcine field sera

A Afshar et al. Can J Vet Res. 1987 Oct.

Abstract

The performance of an indirect enzyme-linked immunosorbent assay and the standard serum neutralization test for detection of antibodies to pseudorabies virus in porcine field sera were compared, using 304 sera from pseudorabies-free pigs in Canada, and 1082 and 580 swine sera from the USA and England, respectively. The sensitivity and specificity of the ELISA relative to the serum neutralization test were in the order of 97 to 98%, with the higher agreement between the tests when a 1:40 dilution of serum samples was used for the ELISA. The indirect ELISA was considered to be a rapid and convenient procedure, offering many advantages over the serum neutralization test for routine serodiagnostic use.

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References

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    1. Can J Comp Med. 1977 Jan;41(1):19-25 - PubMed
    1. N Z Vet J. 1985 Aug;33(8):132-5 - PubMed
    1. Vet Rec. 1983 Jul 9;113(2):38-41 - PubMed
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