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. 2018 Jan;32(1):e22217.
doi: 10.1002/jcla.22217. Epub 2017 Apr 10.

Determination of irinotecan, SN-38 and SN-38 glucuronide using HPLC/MS/MS: Application in a clinical pharmacokinetic and personalized medicine in colorectal cancer patients

Chalirmporn Atasilp  1   2 , Pichai Chansriwong  3 , Ekapob Sirachainan  3 , Thanyanan Reungwetwattana  3 , Apichaya Puangpetch  1   2 , Santirhat Prommas  1   2 , Suwannee Sirilerttrakul  3 , Budsaba Rerkarmnuaychoke  4 , Sansanee Wongwaisayawan  5 , Chonlaphat Sukasem  1   2
Affiliations

Determination of irinotecan, SN-38 and SN-38 glucuronide using HPLC/MS/MS: Application in a clinical pharmacokinetic and personalized medicine in colorectal cancer patients

Chalirmporn Atasilp et al. J Clin Lab Anal. 2018 Jan.

Abstract

Background: Irinotecan (CPT-11) is chemotherapy used mainly in the metastatic colorectal cancer. The purpose of this study was to develop and validate the LC-MS/MS for the simultaneous determination of CPT-11, SN-38, and SN-38G.

Methods: A 100 μL of plasma was prepared after protein precipitation and analyzed on a C18 column using 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile as mobile phases. The mass spectrometer worked with multiple reaction monitoring (MRM) in positive scan mode. The standard curves were linear on a concentration range of 5-10 000 ng/mL for CPT-11, 5-1000 ng/mL for SN-38, and 8-1000 ng/mL for SN-38G.

Results: In this assay, the intra and interday precision consisted of ≤9.11% and ≤11.29% for CPT-11, ≤8.70% and 8.31% for SN-38, and ≤9.90 and 9.64% for SN-38G.

Conclusion: This method was successfully used to quantify CPT-11, SN-38, and SN-38G and applied to a pharmacokinetic study.

Keywords: CPT-11; LC-MS/MS; SN-38; SN-38G; colorectal cancer.

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Figures

Figure 1
Figure 1
The plasma concentration time curves of CPT‐11, SN‐38, and SN‐38G observed in patients (N=4) receiving 180 mg/m2 of CPT‐11 in 90 minutes
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