Review
doi: 10.1016/j.copbio.2017.03.012.
Epub 2017 Apr 7.
Fluorescent labelling in living cells
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- PMID: 28395178
- DOI: 10.1016/j.copbio.2017.03.012
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Review
Fluorescent labelling in living cells
Curr Opin Biotechnol.
2017 Dec.
Abstract
The labelling of proteins with green fluorescent protein enabled the visualization of proteins in living cells for the first time. Since then, much progress has been made in the field. Modern strategies allow the labelling of proteins in live cells through a range of specialized methods with sophisticated chemical probes that show enhanced photophysical properties compared to fluorescent proteins. This review briefly summarizes recent advances in the field of fluorescent chemical protein labelling inside living cells and illustrates key aspects on the requirements and advantages of each given method.
Copyright © 2017 Elsevier Ltd. All rights reserved.
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