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Review
. 2017 Dec:48:61-68.
doi: 10.1016/j.copbio.2017.03.012. Epub 2017 Apr 7.

Fluorescent labelling in living cells

Anselm Fabian Lowell Schneider  1 , Christian Peter Richard Hackenberger  2
Affiliations
Review

Fluorescent labelling in living cells

Anselm Fabian Lowell Schneider et al. Curr Opin Biotechnol. 2017 Dec.

Abstract

The labelling of proteins with green fluorescent protein enabled the visualization of proteins in living cells for the first time. Since then, much progress has been made in the field. Modern strategies allow the labelling of proteins in live cells through a range of specialized methods with sophisticated chemical probes that show enhanced photophysical properties compared to fluorescent proteins. This review briefly summarizes recent advances in the field of fluorescent chemical protein labelling inside living cells and illustrates key aspects on the requirements and advantages of each given method.

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