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. 2017 Aug 15;33(16):2583-2585.
doi: 10.1093/bioinformatics/btx198.

MISA-web: a web server for microsatellite prediction

Affiliations

MISA-web: a web server for microsatellite prediction

Sebastian Beier et al. Bioinformatics. .

Abstract

Motivation: Microsatellites are a widely-used marker system in plant genetics and forensics. The development of reliable microsatellite markers from resequencing data is challenging.

Results: We extended MISA, a computational tool assisting the development of microsatellite markers, and reimplemented it as a web-based application. We improved compound microsatellite detection and added the possibility to display and export MISA results in GFF3 format for downstream analysis.

Availability and implementation: MISA-web can be accessed under http://misaweb.ipk-gatersleben.de/. The website provides tutorials, usage note as well as download links to the source code.

Contact: scholz@ipk-gatersleben.de.

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Figures

Fig. 1
Fig. 1
MISA-web analysis workflow. MISA-web was updated and set up as a web-application on the IPK server. Users may either paste their nucleotide sequence of interest in the input fields of MISA-web or supply accession numbers to have the corresponding sequences fetched from NCBI (1). Once all input fields have been filled (2), a click on the start button on the bottom of the page starts the analysis. The computation will be conducted on a compute server (3) and the result files will be sent to a user-specified email address (4). Result files can be examined afterwards (5)

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