Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 May;32(5):740-6.
doi: 10.1128/AAC.32.5.740.

Effect of aminoglycoside concentration on reaction rates of aminoglycoside-modifying enzymes

G P Bongaerts  1 , J S Vliegenthart
Affiliations

Effect of aminoglycoside concentration on reaction rates of aminoglycoside-modifying enzymes

G P Bongaerts et al. Antimicrob Agents Chemother. 1988 May.

Abstract

Reaction rates of several reference aminoglycoside-modifying enzymes were studied at various substrate concentrations. The resulting concentration-response curves showed wide variation in threshold concentration, in curve slope, in enzyme saturation, and in substrate inhibition. Together, the curves of a defined aminoglycoside panel yielded more specific information for each individual aminoglycoside-modifying enzyme tested than did conventional substrate profiles obtained at a single substrate concentration.

PubMed Disclaimer

References

    1. Biochemistry. 1971 May 11;10(10):1787-96 - PubMed
    1. Antimicrob Agents Chemother. 1974 Oct;6(4):492-7 - PubMed
    1. Methods Enzymol. 1975;43:611-28 - PubMed
    1. Antimicrob Agents Chemother. 1976 Jun;9(6):945-50 - PubMed
    1. Antimicrob Agents Chemother. 1976 Jun;9(6):951-5 - PubMed