Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Jul;31(7):1648-1650.
doi: 10.1038/leu.2017.115. Epub 2017 Apr 12.

NEK2 induces osteoclast differentiation and bone destruction via heparanase in multiple myeloma

M Hao  1   2 , R Franqui-Machin  2   3 , H Xu  2 , J Shaughnessy Jr  4 , B Barlogie  4 , D Roodman  5 , D E Quelle  6 , S Janz  7 , M H Tomasson  2 , R D Sanderson  8 , L Qiu  1 , I Frech  2 , G Tricot  2 , F Zhan  2   3
Affiliations

NEK2 induces osteoclast differentiation and bone destruction via heparanase in multiple myeloma

M Hao et al. Leukemia. 2017 Jul.
No abstract available

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
High expression of NEK2 in MM cells is positively correlated with bone lytic lesions in newly diagnosed MM patients. (a) Bar-view represents the correlation of NEK2 gene expression in MM cells from 89 patients without bone lytic lesion and 155 patients with one or more bone lesions on FDG-PET scan. (b) Bar-view represents the correlation of NEK2 gene expression in MM cells from 74 patients without bone lytic lesion and 174 patients with one or more bone lesions on MRI. (c) Box-plots show the expression of NEK2 and HPSE in 50 samples with high-NEK2 and low-NEK2.
Figure 2
Figure 2
Overexpression of NEK2 in MM cells enhances osteoclast differentiation and bone destruction. (a) Human bone marrow macrophages (BMMs) from MM patients were cultured in the conditioned media collected from the control (EV), NEK2 OE and NEK2 shRNA ARP1 and OCI-MY5 lines for 14 days. Osteoclasts were detected by TRAP staining. (b) BMMs were cultured in the conditioned media collected from the control (EV), NEK2 OE, and NEK2 OE-shRNA HPSE (NEK2 OE shHPSE) ARP1 and OCI-MY5 lines for 14 days. Osteoclasts were detected by TRAP staining. (c) Representative X-ray, μCT and histological images for H&E and TRAP staining of bones from mice treated with ARP1 EV, ARP1 NEK2 OE and NEK2 OE plus Roneparstat (NEK2 OE SST). Red arrows indicate bone lytic lesions by X-ray.
See this image and copyright information in PMC

References

    1. Kuehl WM, Bergsagel PL. Multiple myeloma: evolving genetic events and host interactions. Nat Rev Cancer 2002; 2: 175–187. - PubMed
    1. Billadeau D, Ahmann G, Greipp P, Van Ness B. The bone marrow of multiple myeloma patients contains B cell populations at different stages of differentiation that are clonally related to the malignant plasma cell. J Exp Med 1993; 178: 1023–1031. - PMC - PubMed
    1. Zhou W, Yang Y, Xia J, Wang H, Salama ME, Xiong W et al. NEK2 induces drug resistance mainly through activation of efflux drug pumps and is associated with poor prognosis in myeloma and other cancers. Cancer Cell 2013; 23: 48–62. - PMC - PubMed
    1. Zhou W, Yang Y, Gu Z, Wang H, Xia J, Wu X et al. ALDH1 activity identifies tumor-initiating cells and links to chromosomal instability signatures in multiple myeloma. Leukemia 2014; 28: 1155–1158. - PMC - PubMed
    1. Faragher AJ, Fry AM. Nek2A kinase stimulates centrosome disjunction and is required for formation of bipolar mitotic spindles. Mol Biol Cell 2003; 14: 2876–2889. - PMC - PubMed

Publication types

MeSH terms