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. 2016;59(5-6):262-266.
doi: 10.1159/000471484. Epub 2017 Apr 13.

Frequent pUL27 Variations in HIV-Infected Patients

Azam Mirarab  1 , Alireza Mohebbi, Abdolvahab Moradi, Naeme Javid, Mohammad Ali Vakili, Alijan Tabarraei
Affiliations

Frequent pUL27 Variations in HIV-Infected Patients

Azam Mirarab et al. Intervirology. 2016.

Abstract

Objective: Drug-resistant isolates of human cytomegalovirus (HCMV) have led to the development of new anti-HCMV drugs. Maribavir (MBV) is a novel inhibitor of the HCMV viral kinase. Resistance to MBV is mapped to gene UL27, a viral nuclear protein. In this study, we investigated UL27 polymorphisms in MBV-naive HIV-positive and HCMV congenitally infected clinical samples.

Methods: DNA was extracted from 20 CMV-positive HIV (9/20) and congenitally infected (11/20) patients and used for UL27 polymerase chain reaction amplification. Sanger sequencing and multiple sequence alignment of products was performed.

Results: K90 was the most prevalent polymorphism in both HIV-positive and congenitally infected patients. Polymorphisms Q54, D123, and R107 (10%) were seen in more than one sample. There were significantly more polymorphisms in the HIV-positive samples (p = 0.038).

Conclusion: HCMV pUL27 is highly variable in adult immunocompromised HIV-positive patients.

Keywords: Congenital cytomegalovirus infection; HIV infection; Human cytomegalovirus; Polymorphism; pUL27.

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