Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

doi:10.1371/journal.pntd.0005538

. 2017 Apr 12;11(4):e0005538.

doi: 10.1371/journal.pntd.0005538. eCollection 2017 Apr.

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Mehmet Karakuş¹, Abed Nasereddin^{2

3}, Hüseyin Onay⁴, Emin Karaca⁴, Ahmet Özkeklikçi⁵, Charles L Jaffe², Katrin Kuhls⁶, Ahmet Özbilgin⁷, Hatice Ertabaklar⁸, Samiye Demir⁹, Yusuf Özbel¹, Seray Töz¹

Affiliations

¹ Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey.
² Department of Microbiology and Molecular Genetics, IMRIC, Hebrew University - Hadassah Medical Center, Jerusalem, Israel.
³ The Core Research Facility, The Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
⁴ Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
⁵ Dr. Ersin Arslan State Hospital, Microbiology Department, Gaziantep, Turkey.
⁶ Technical University of Applied Sciences Wildau, Division of Molecular Biotechnology and Functional Genetics, Wildau, Germany.
⁷ Celal Bayar University, Faculty of Medicine, Department of Parasitology, Manisa, Turkey.
⁸ Adnan Menderes University, Faculty of Medicine, Department of Parasitology, Aydın, Turkey.
⁹ Ege University, Faculty of Science, Department of Biology, Izmir, Turkey.

PMID: 28403153
PMCID: PMC5402985
DOI: 10.1371/journal.pntd.0005538

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Mehmet Karakuş et al. PLoS Negl Trop Dis. 2017.

. 2017 Apr 12;11(4):e0005538.

doi: 10.1371/journal.pntd.0005538. eCollection 2017 Apr.

Authors

Affiliations

¹ Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey.
² Department of Microbiology and Molecular Genetics, IMRIC, Hebrew University - Hadassah Medical Center, Jerusalem, Israel.
³ The Core Research Facility, The Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel.
⁴ Ege University, Faculty of Medicine, Department of Medical Genetics, Izmir, Turkey.
⁵ Dr. Ersin Arslan State Hospital, Microbiology Department, Gaziantep, Turkey.
⁶ Technical University of Applied Sciences Wildau, Division of Molecular Biotechnology and Functional Genetics, Wildau, Germany.
⁷ Celal Bayar University, Faculty of Medicine, Department of Parasitology, Manisa, Turkey.
⁸ Adnan Menderes University, Faculty of Medicine, Department of Parasitology, Aydın, Turkey.
⁹ Ege University, Faculty of Science, Department of Biology, Izmir, Turkey.

PMID: 28403153
PMCID: PMC5402985
DOI: 10.1371/journal.pntd.0005538

Abstract

Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Şanlıurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Şanlıurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

**Fig 1. STRUCTURE analysis of the L. *tropica* samples.**

**Fig 2. Geographical distribution of MLMT profiles.**

**Fig 3. STRUCTURE analysis of the Turkish L. *tropica* samples (including previous L. *tropica* data).**

See this image and copyright information in PMC

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[1] Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7(5). - PMC - PubMed

[2] Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, et al. Leishmaniasis worldwide and global estimates of its incidence. PLoS One. 2012;7(5). - PMC - PubMed

[3] Gürel MS, Yeşilova Y, Olgen MK, Ozbel Y. Cutaneous leishmaniasis in Turkey. Turkiye Parazitol Derg. 2012;36(2):121–9. 10.5152/tpd.2012.29 - DOI - PubMed

[4] Gürel MS, Yeşilova Y, Olgen MK, Ozbel Y. Cutaneous leishmaniasis in Turkey. Turkiye Parazitol Derg. 2012;36(2):121–9. 10.5152/tpd.2012.29 - DOI - PubMed

[5] Özbilgin A, Çulha G, Uzun S, Harman M, Topal SG, Okudan F, et al. Leishmaniasis in Turkey: first clinical isolation of Leishmania major from 18autochthonous cases of cutaneous leishmaniasis in four geographical regions. Trop. Med. Int. Health 2016; 21 (6), 783–791, 10.1111/tmi.12698. - DOI - PubMed

[6] Özbilgin A, Çulha G, Uzun S, Harman M, Topal SG, Okudan F, et al. Leishmaniasis in Turkey: first clinical isolation of Leishmania major from 18autochthonous cases of cutaneous leishmaniasis in four geographical regions. Trop. Med. Int. Health 2016; 21 (6), 783–791, 10.1111/tmi.12698. - DOI - PubMed

[7] Svobodová M, Alten B, Zídková L, Dvorák V, Hlavacková J, Mysková J, et al. Cutaneous leishmaniasis caused by Leishmania infantum transmitted by Phlebotomus tobbi. Int J Parasitol. 2009. January;39(2):251–6. 10.1016/j.ijpara.2008.06.016 - DOI - PubMed

[8] Svobodová M, Alten B, Zídková L, Dvorák V, Hlavacková J, Mysková J, et al. Cutaneous leishmaniasis caused by Leishmania infantum transmitted by Phlebotomus tobbi. Int J Parasitol. 2009. January;39(2):251–6. 10.1016/j.ijpara.2008.06.016 - DOI - PubMed

[9] Ozensoy Toz S, Culha G, Zeyrek FY, Ertabaklar H, Alkan MZ, Vardarlı AT, et al. A real-time ITS1-PCR based method in the diagnosis and species identification of Leishmania parasite from human and dog clinical samples in Turkey. PLoS Negl Trop Dis. 2013. May 9;7(5):e2205 10.1371/journal.pntd.0002205 - DOI - PMC - PubMed

[10] Ozensoy Toz S, Culha G, Zeyrek FY, Ertabaklar H, Alkan MZ, Vardarlı AT, et al. A real-time ITS1-PCR based method in the diagnosis and species identification of Leishmania parasite from human and dog clinical samples in Turkey. PLoS Negl Trop Dis. 2013. May 9;7(5):e2205 10.1371/journal.pntd.0002205 - DOI - PMC - PubMed

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Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Affiliations

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Authors

Affiliations

Abstract

Conflict of interest statement

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