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. 2017 Apr 12;11(4):e0005538.
doi: 10.1371/journal.pntd.0005538. eCollection 2017 Apr.

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Mehmet Karakuş  1 , Abed Nasereddin  2   3 , Hüseyin Onay  4 , Emin Karaca  4 , Ahmet Özkeklikçi  5 , Charles L Jaffe  2 , Katrin Kuhls  6 , Ahmet Özbilgin  7 , Hatice Ertabaklar  8 , Samiye Demir  9 , Yusuf Özbel  1 , Seray Töz  1
Affiliations

Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT)

Mehmet Karakuş et al. PLoS Negl Trop Dis. .

Abstract

Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Şanlıurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Şanlıurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies.

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Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. STRUCTURE analysis of the L. tropica samples.
Fig 2
Fig 2. Geographical distribution of MLMT profiles.
Fig 3
Fig 3. STRUCTURE analysis of the Turkish L. tropica samples (including previous L. tropica data).
See this image and copyright information in PMC

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