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Multicenter Study
. 2017 Jun;55(6):1954-1960.
doi: 10.1128/JCM.00244-17. Epub 2017 Apr 12.

Multicenter Performance Assessment of Carba NP Test

Scott A Cunningham  1 , Brandi Limbago  2 , Maria Traczewski  3 , Karen Anderson  2 , Meredith Hackel  4 , Janet Hindler  5 , Dan Sahm  4 , Efe Alyanak  2 , Adrian Lawsin  2 , Christopher A Gulvik  2 , Tom J B de Man  2 , Jayawant N Mandrekar  6 , Audrey N Schuetz  1   7 , Stephen Jenkins  7 , Romney Humphries  5 , Elizabeth Palavecino  8 , Shawn Vasoo  1   9   10 , Robin Patel  11   10
Affiliations
Multicenter Study

Multicenter Performance Assessment of Carba NP Test

Scott A Cunningham et al. J Clin Microbiol. 2017 Jun.

Abstract

Eighty Gram-negative bacilli (54 Enterobacteriaceae and 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0.05). Previously described limitations with blaOXA-48-like carbapenemases and blaOXA carbapenemases associated with Acinetobacter baumannii were noted. Based on these data, we demonstrate that the Carba NP test, when implemented with the standardized CLSI methodology, provides reproducible results across multiple sites for detection of carbapenemases.

Keywords: Gram-negative bacilli; antimicrobial resistance; carbapenemase; screening.

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References

    1. Escamilla J. 1976. Susceptibility of Haemophilus influenzae to ampicillin as determined by use of a modified, one-minute beta-lactamase test. Antimicrob Agents Chemother 9:196–198. doi:10.1128/AAC.9.1.196. - DOI - PMC - PubMed
    1. Skinner A, Wise R. 1977. A comparison of three rapid methods for the detection of beta-lactamase activity in Haemophilus influenzae. J Clin Pathol 30:1030–1032. doi:10.1136/jcp.30.11.1030. - DOI - PMC - PubMed
    1. Vasoo S, Cunningham SA, Kohner PC, Simner PJ, Mandrekar JN, Lolans K, Hayden MK, Patel R. 2013. Comparison of a novel, rapid chromogenic biochemical assay, the Carba NP test, with the modified Hodge test for detection of carbapenemase-producing Gram-negative bacilli. J Clin Microbiol 51:3097–3101. doi:10.1128/JCM.00965-13. - DOI - PMC - PubMed
    1. Tijet N, Boyd D, Patel SN, Mulvey MR, Melano RG. 2013. Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother 57:4578–4580. doi:10.1128/AAC.00878-13. - DOI - PMC - PubMed
    1. Lee LY, Korman TM, Graham M. 2014. Rapid time to results and high sensitivity of the CarbaNP test on early cultures. J Clin Microbiol 52:4023–4026. doi:10.1128/JCM.01682-14. - DOI - PMC - PubMed

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