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. 2017 Apr 13:23:1783-1791.
doi: 10.12659/msm.900471.

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Jie Chen  1 , Wan-Chen Li  1 , Xin-Li Gu  1
Affiliations

Optimized Extraction, Preliminary Characterization, and In Vitro Antioxidant Activity of Polysaccharides from Glycyrrhiza Uralensis Fisch

Jie Chen et al. Med Sci Monit. .

Abstract

BACKGROUND This study performed optimized extraction, preliminary characterization, and in vitro antioxidant activities of polysaccharides from Glycyrrhiza uralensis Fisch. MATERIAL AND METHODS Three parameters (extraction temperature, ratio of water to raw material, and extraction time) were optimized for yields of G. uralensis polysaccharides (GUP) using response surface methodology with Box-Behnken design (BBD). The GUP was purified using DEAE cellulose 32-column chromatography. The main fraction obtained from G. uralensis Fisch was GUP-II, which was composed of rhamnose, arabinose, galactose, and glucose monosaccharide, was screened for antioxidant properties using DP Hand hydroxyl radical scavenging assays. In addition, immunological activity of GUP-II was determined by nitric oxide and lymphocyte proliferation assays. RESULTS Optimization revealed maximum GUP yields with an extraction temperature of 99°C, water: raw material ratio of 15: 1, and extraction duration of 2 h. GUP-II purified from G. uralensis Fisch had good in vitro DPPH and hydroxyl radical scavenging abilities. Immunologically, GUP-II significantly stimulated NO production in RAW 264.7 macrophages, and significantly enhanced LPS-induced lymphocyte proliferation. CONCLUSIONS Extraction of GUP from G. uralensis Fisch can be optimized with respect to temperature, extraction period, and ratio of water to material, using response surface methodology. The purified product (GUP-II) possesses excellent antioxidant and immunological activities.

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Conflict of interest statement

Conflict of interest

The authors declare there no conflict of interest.

Figures

Figure 1
Figure 1
3D response surface plots (A, C, E) and 2D contour plots (B, D, F) determined the effects of extraction temperature, ratio of water to raw material, and extraction time, respectively, at 3 different levels on the yield of G. uralensis polysaccharide.
Figure 2
Figure 2
Purification and analysis of GUP-II from GUP. (A) GUP-I, GUP-II, GUP-III, GUP-IV and GUP-V were fractionated from the crude GUP by DEAE cellulose-32 chromatography. (B) GUP-II yielded a single peak, which was further purified by a Sephadex G-100 column. (C, D) GUP-II was composed of arabinose, rhamnose, galactose, and glucose as evidenced by HPAEC analysis (C – standard control; D – GUP-II).
Figure 3
Figure 3
DPPH scavenging activity of GUP-II and BHT.
Figure 4
Figure 4
Hydroxyl radical scavenging activities of GUP-II and vitamin C
Figure 5
Figure 5
Effects of GUP-II on macrophage activation depended on NO production in RAW264.7 cells. (A: Different concentrations; B: Different time points). * p<0.05 compared with control.
Figure 6
Figure 6
Effect of GUP-II on T and B cell proliferation detected and verified by MTT. * p<0.05 compared with control.
See this image and copyright information in PMC

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