Resonance assignments of non-exchangeable protons in B type DNA oligomers, an overview
- PMID: 2840632
- PMCID: PMC336824
- DOI: 10.1093/nar/16.13.5713
Resonance assignments of non-exchangeable protons in B type DNA oligomers, an overview
Abstract
The chemical shifts of 1H resonances of non exchangeable protons (except H5', H5" and adenine H2) of over six hundred nucleotides have been collected. The influence which the base of the nucleotide itself as well as the bases on its 5' and 3' side exert on the chemical shifts of the various resonances has been investigated. Most of the resonances appear to be predominantly influenced by only one base. For H2', H2", H3', H4' and H6/H8 this is the base of the central nucleotide, for H5(C) and CH3(T) it is the one on the 5' side and for H1' it is the one on the 3' side. Chemical shift distribution profiles are presented which allow an estimation of the probability of finding a particular resonance at a particular position in the spectrum.
Similar articles
-
Determination of nucleic acid backbone conformation by 1H NMR.Biochemistry. 1992 Apr 14;31(14):3564-74. doi: 10.1021/bi00129a003. Biochemistry. 1992. PMID: 1373647
-
Sequential resonance assignments in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy.Biochemistry. 1984 Mar 27;23(7):1371-6. doi: 10.1021/bi00302a006. Biochemistry. 1984. PMID: 6722097
-
The identification of the A-type RNA helices in a 55mer RNA by selective incorporation of deuterium-labelled nucleotide residues (Uppsala NMR-window concept).J Biochem Biophys Methods. 2000 Mar 16;42(3):153-78. doi: 10.1016/s0165-022x(99)00057-3. J Biochem Biophys Methods. 2000. PMID: 10737221
-
Conformational analysis of a hybrid DNA-RNA double helical oligonucleotide in aqueous solution: d(CG)r(CG)d(CG) studied by 1D- and 2D-1H NMR spectroscopy.J Biomol Struct Dyn. 1983 Oct;1(1):131-49. doi: 10.1080/07391102.1983.10507430. J Biomol Struct Dyn. 1983. PMID: 6086057 Review.
-
1H NMR spectroscopy of DNA.Methods Enzymol. 1992;211:235-53. doi: 10.1016/0076-6879(92)11015-b. Methods Enzymol. 1992. PMID: 1328824 Review. No abstract available.
Cited by
-
Calibration of ring-current effects in proteins and nucleic acids.J Biomol NMR. 1995 Dec;6(4):341-6. doi: 10.1007/BF00197633. J Biomol NMR. 1995. PMID: 8563464
-
N.m.r. assignments and temperature-dependent conformational transitions of a mutant trp operator-promoter in solution.Biochem J. 1989 May 1;259(3):715-24. doi: 10.1042/bj2590715. Biochem J. 1989. PMID: 2730583 Free PMC article.
-
The role of the phosphorus BI-BII transition in protein-DNA recognition: the NF-kappaB complex.Nucleic Acids Res. 2002 Oct 15;30(20):4452-9. doi: 10.1093/nar/gkf559. Nucleic Acids Res. 2002. PMID: 12384592 Free PMC article.
-
Prediction of proton chemical shifts in RNA. Their use in structure refinement and validation.J Biomol NMR. 2001 Sep;21(1):11-29. doi: 10.1023/a:1011914132531. J Biomol NMR. 2001. PMID: 11693565
-
Highly diastereoselective synthesis of nucleoside adducts from the carcinogenic benzo[a]pyrene diol epoxide and a computational analysis.J Am Chem Soc. 2007 Jan 10;129(1):68-76. doi: 10.1021/ja063902u. J Am Chem Soc. 2007. PMID: 17199284 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
