Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Feb;26(1):291-301.
doi: 10.1007/s10787-017-0345-0. Epub 2017 Apr 13.

Anti-inflammatory activity of the functional groups present in Hippophae rhamnoides (Seabuckthorn) leaf extract

Himanshi Tanwar  1 , Shweta  1 , Divya Singh  1 , Shashi Bala Singh  1 , Lilly Ganju  2
Affiliations

Anti-inflammatory activity of the functional groups present in Hippophae rhamnoides (Seabuckthorn) leaf extract

Himanshi Tanwar et al. Inflammopharmacology. 2018 Feb.

Erratum in

Abstract

Ethno pharmacological relevance: The study explores the anti-inflammatory activity of components present in fractions obtained from leaves of Hippophae rhamnoides in mouse peritoneal macrophages.

Aim of the study: Immunomodulators salvage the immune response by enhancing or reducing its capacity to the required level. Plant extracts are extensively used as immunomodulators because of their easy availability, simple methods of preparation and minimum side effects with maximum efficacy.

Materials and methods: The present study was conducted to assess the immunomodulatory activities of phyto constituents present in Seabuckthorn leaves. The aqueous-alcoholic leaf extract was subjected to successive and parallel extraction in the presence of polar and non-polar solvents for fractionation of compounds. Based on the yield, three fractions were selected viz. parallel methanol (PM), successive chloroform (SC) and successive methanol (SM) and screened for in vitro immunomodulatory activities. Peritoneal macrophages were isolated from Balb/c mice and cultured with or without LPS to evaluate the immunomodulatory effect of the three fractions on cell viability, hemolytic activity, nitric oxide (NO) production, cytokine levels, iNOS and COX-2 expressions.

Results: The results revealed that none of the three fractions induced hemolysis. Cells treated with PM fraction significantly suppressed LPS-induced NO production and pro-inflammatory cytokines such as TNF-α, IL-6 and IFN-γ as compared to SC and SM treatment. The iNOS and COX-2 expressions were also significantly reduced after treatment with PM fraction.

Conclusions: The decrease in LPS-induced NO production, pro-inflammatory cytokine secretion, iNOS and COX-2 expression signifies anti-inflammatory properties of PM fraction containing tannins, proteins and carbohydrate groups. Hence, this plant-derived immunomodulator can be used as a therapeutic agent in inflammatory diseases.

Keywords: Anti-inflammatory property; Immunomodulation; Nitric oxide; Pro-inflammatory cytokines.

PubMed Disclaimer

References

    1. Eur J Med Chem. 2013 May;63:570-7 - PubMed
    1. Curr Med Chem. 2004 Jun;11(11):1451-60 - PubMed
    1. J Pharm Pharmacol. 2007 Dec;59(12 ):1739-45 - PubMed
    1. J Leukoc Biol. 2000 Oct;68(4):553-60 - PubMed
    1. Int Immunopharmacol. 2006 Jan;6(1):46-52 - PubMed

MeSH terms

LinkOut - more resources