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. 2017 Dec;26(6):331-337.
doi: 10.1080/13645706.2017.1310737. Epub 2017 Apr 18.

Changes in peripheral blood T-cell balance after percutaneous tumor ablation

Haruyuki Takaki  1 , Naoko Imai  2 , Contessa T Thomas  3 , Koichiro Yamakado  1 , Hooman Yarmohammadi  3 , Etay Ziv  3 , Govindarajan Srimathveeravalli  3 , Constantinos T Sofocleous  3 , Stephen B Solomon  3 , Joseph P Erinjeri  3
Affiliations

Changes in peripheral blood T-cell balance after percutaneous tumor ablation

Haruyuki Takaki et al. Minim Invasive Ther Allied Technol. 2017 Dec.

Abstract

Purpose: To evaluate the changes in T-cell balance in peripheral blood following percutaneous tumor ablation.

Material and methods: Patients underwent thermal ablation including radiofrequency (n = 9) and microwave ablation (n = 5), or cryoablation (n = 5). Target tumors were located in the lung (n = 7), soft tissue (n = 5), liver (n = 4), and bone (n = 3). Patient peripheral blood samples were collected before and within 14 days after ablation. Peripheral blood populations of cytotoxic T-cells (CTL), type-1 (Th1) and type-2 helper T-cells (Th2), and regulatory T-cells (Treg) were measured using flow cytometry. Changes in CTL/Treg and Th1/Th2 ratios before and after ablation therapy were compared using paired t-tests.

Results: Peripheral blood CTL population (27.5 ± 2.1% to 30.2 ± 2.5%, p < .03) and CTL/Treg ratios (18.8 ± 3.7% to 21.6 ± 3.6%, p < .05) increased significantly after ablation. Although a significant increase in CTL/Treg ratios was found after heat-based ablation (18.0 ± 4.4% to 21.6 ± 4.7%, p < .02), it remained unchanged after cryoablation (21.0 ± 7.0% to 21.5 ± 4.3%, p = .92). Th1/Th2 ratio (13.7 ± 3.0% to 17.2 ± 3.5%, p = .12) remained unchanged after ablation.

Conclusion: Ablation therapy alters the T-cell balance by increasing the systemic CTL/Treg, ratio. Heat-based ablation might be a more effective approach than cryoablation to enhance systemic anti-tumor immunity.

Keywords: Radiofrequency ablation; T-cell; cryoablation; lymphocyte; microwave ablation.

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Conflict of interest statement

Disclosure statement

The authors report no conflict of interest.

Figures

Figure 1.
Figure 1.
Representative flow cytometry results for a patient who received heat-based ablation for the treatment of lung metastasis from colorectal cancer. (a) Population of peripheral blood cytotoxic T-lymphocyte (CTL) changed from 35.1% to 39.2% at three days after ablation. (b) The CTL/regulatory T cell (Treg) ratio increased from 35.0% to 43.3% after ablation.(c) In this case, type-1 (Th1)/type-2 helper T-cells (Th2) ratio also increased 6.6% to 7.3% after ablation.
See this image and copyright information in PMC

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