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. 2017 Apr 4:11:176.
doi: 10.3389/fnins.2017.00176. eCollection 2017.

Neuropeptide Y-Induced Orexigenic Action Is Attenuated by the Orexin Receptor Antagonist in Bullfrog Larvae

Kouhei Matsuda  1   2 , Kairi Matsumura  1 , Syun-Suke Shimizu  1 , Tomoya Nakamachi  1 , Norifumi Konno  1
Affiliations

Neuropeptide Y-Induced Orexigenic Action Is Attenuated by the Orexin Receptor Antagonist in Bullfrog Larvae

Kouhei Matsuda et al. Front Neurosci. .

Abstract

In bullfrog larvae at the pre- and pro-metamorphic stages, feeding behavior is regulated by appetite factors such as orexigenic peptides. In fact, food intake is enhanced by intracerebroventricular (ICV) administration of neuropeptide Y (NPY) and orexin A. Using goldfish, our previous study indicated that the orexigenic action of NPY is mediated by orexin A, suggesting the functional interaction between the two. However, there is little information about whether the action of orexin A mediates the orexigenic action of NPY in bullfrog larvae. Therefore, we examined the effect of the orexin receptor antagonist, SB334867 on the orexigenic action of NPY in larvae. The stimulatory effect of ICV injection of NPY at 10 pmol/g body weight (BW) on food intake was abolished by treatment with SB334867 at 60 pmol/g BW. These results suggest that, in bullfrog larvae, there is a neuronal relationship between the NPY and orexin systems, and that the orexigenic action of NPY is mediated by the orexin A-induced orexigenic action.

Keywords: NPY; antagonist; bullfrog larvae; neuronal interaction; orexigenic action; orexin A.

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Figures

Figure 1
Figure 1
Effect of ICV administration of a NPY Y1-receptor antagonist, BIBP3226, on the orexigenic action of NPY in prometamorphic larvae. Each column and bar represents the mean and SEM, respectively, and the numbers in parentheses in the panels indicate the number of larvae in each group. Significance of differences was evaluated by two-way ANOVA with Bonferroni's method. Different superscripts (a and b) indicate statistical significance (P < 0.05).
Figure 2
Figure 2
Effect of ICV administration of an orexin receptor antagonist, SB334867, on the orexigenic action of orexin A in prometamorphic larvae. Each column and bar represents the mean and SEM, respectively, and the numbers in parentheses in the panels indicate the number of larvae in each group. Significance of differences was evaluated by two-way ANOVA with Bonferroni's method. Different superscripts (a and b) indicate statistical significance (P < 0.05).
Figure 3
Figure 3
Effect of ICV administration of SB334867 on the orexigenic action of NPY in prometamorphic larvae. Each column and bar represents the mean and SEM, respectively, and the numbers in parentheses in the panel indicate the number of larvae in each group. Significance of differences was evaluated by two-way ANOVA with Bonferroni's method. Different superscripts (a and b) indicate statistical significance (P < 0.05).
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