doi: 10.1016/0378-1119(88)90468-4.
Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells
Affiliations
- PMID: 2842234
- DOI: 10.1016/0378-1119(88)90468-4
Item in Clipboard
Plasmid vectors with multiple cloning sites and cat-reporter gene for promoter cloning and analysis in animal cells
Gene.
.
Abstract
We have constructed two vectors, pGCAT-A and pGCAT-C, designed to facilitate the construction of recombinant plasmids containing the bacterial gene (cat) coding for chloramphenicol acetyltransferase (CAT) under the control of eukaryotic promoter and/or enhancer elements. The cat gene was inserted downstream from a multiple cloning site (MCS) region with eleven unique restriction sites. The MCS region is in opposite orientation in the two vectors. The CAT activity of control extracts from cells transfected with pGCAT-A or pGCAT-C is very low. Insertion of the viral SV40 early promoter into one of the sites of the MCS region of pGCAT-A or pGCAT-C results in a 30- to 400-fold stimulation of the CAT activity.
Similar articles
-
Activity of the simian virus 40 early promoter-enhancer in herpes simplex virus type 1 vectors is dependent on its position, the infected cell type, and the presence of Vmw175.J Virol. 1991 Dec;65(12):6900-12. doi: 10.1128/JVI.65.12.6900-6912.1991. J Virol. 1991. PMID: 1658381 Free PMC article.
-
Expression of the chloramphenicol acetyltransferase gene in mammalian cells under the control of adenovirus type 12 promoters: effect of promoter methylation on gene expression.Proc Natl Acad Sci U S A. 1983 Dec;80(24):7586-90. doi: 10.1073/pnas.80.24.7586. Proc Natl Acad Sci U S A. 1983. PMID: 6324179 Free PMC article.
-
A matched set of cat vectors for rapid mutational analysis of eukaryotic promoters and enhancers.Gene. 1991 Aug 30;105(1):97-100. doi: 10.1016/0378-1119(91)90518-g. Gene. 1991. PMID: 1657717
-
In vitro promoter analysis using nuclear extracts and G-free cassette vectors.Methods Mol Biol. 2000;130:247-56. doi: 10.1385/1-59259-686-x:247. Methods Mol Biol. 2000. PMID: 10589436 Review. No abstract available.
-
High-level expression of foreign genes in mammalian cells.Genet Eng (N Y). 1991;13:167-82. doi: 10.1007/978-1-4615-3760-1_7. Genet Eng (N Y). 1991. PMID: 1367413 Review. No abstract available.
Cited by
-
H2O2 and antioxidants have opposite effects on activation of NF-kappa B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor.EMBO J. 1993 May;12(5):2005-15. doi: 10.1002/j.1460-2075.1993.tb05850.x. EMBO J. 1993. PMID: 8491191 Free PMC article.
-
The role of the 5'-flanking region in the cell-specific transcription of the human von Willebrand factor gene.Biochem J. 1993 Aug 1;293 ( Pt 3)(Pt 3):641-8. doi: 10.1042/bj2930641. Biochem J. 1993. PMID: 8352730 Free PMC article.
-
An Sp1 binding site and the minimal promoter contribute to overexpression of the cytokeratin 18 gene in tumorigenic clones relative to that in nontumorigenic clones of a human carcinoma cell line.Mol Cell Biol. 1995 May;15(5):2490-9. doi: 10.1128/MCB.15.5.2490. Mol Cell Biol. 1995. PMID: 7537848 Free PMC article.
-
DNA recognition by splicing variants of the Wilms' tumor suppressor, WT1.Mol Cell Biol. 1994 Jun;14(6):3800-9. doi: 10.1128/mcb.14.6.3800-3809.1994. Mol Cell Biol. 1994. PMID: 8196623 Free PMC article.
-
Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene.Proc Natl Acad Sci U S A. 1994 Jul 19;91(15):7202-6. doi: 10.1073/pnas.91.15.7202. Proc Natl Acad Sci U S A. 1994. PMID: 7913748 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Miscellaneous
