doi: 10.1128/jb.170.9.4382-4384.1988.
Cloning of the Rhodobacter capsulatus hemA gene
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- PMID: 2842318
- PMCID: PMC211455
- DOI: 10.1128/jb.170.9.4382-4384.1988
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Cloning of the Rhodobacter capsulatus hemA gene
J Bacteriol.
1988 Sep.
Abstract
Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment.
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