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. 1988 Sep;170(9):4382-4.
doi: 10.1128/jb.170.9.4382-4384.1988.

Cloning of the Rhodobacter capsulatus hemA gene

S W Biel  1 , M S Wright, A J Biel
Affiliations

Cloning of the Rhodobacter capsulatus hemA gene

S W Biel et al. J Bacteriol. 1988 Sep.

Abstract

Portions of the Rhodobacter capsulatus hemA gene have been cloned from a hemA::Tn5 insertion strain into the lambda bacteriophage derivative EMBL3. A cosmid containing the wild-type R. capsulatus hemA gene was isolated by complementation of the hemA::Tn5 mutant. The cosmid contains a 1.4-kilobase EcoRI fragment that spans the hemA::Tn5 insertion site. The entire hemA gene is contained in this fragment and the adjacent 0.6-kilobase EcoRI fragment.

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References

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